PMID- 10586086
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20100825
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 12
DP  - 1999 Dec 15
TI  - The rat and mouse homologues of MASP-2 and MAp19, components of the lectin
      activation pathway of complement.
PG  - 6848-59
AB  - Recently, we described two novel constituents of the multimolecular initiation
      complex of the mannan-binding lectin (MBL) pathway of complement activation, a
      serine protease of 76 kDa, termed MASP-2, and a MASP-2 related plasma protein of 
      19 kDa, termed MAp19. Upon activation of the MBL/MASPs/MAp19 complex, MASP-2
      cleaves the fourth complement component C4, while the role of MAp19 within the
      MBL/MASP-1/MASP-2/MAp19 complex remains to be clarified. In humans, the mRNA
      species encoding MASP-2 (2.6 kb) and MAp19 (1.0 kb) arise by an alternative
      polyadenylation/splicing mechanism from a single structural MASP-2 gene. Here, we
      report the complete primary structures of the rat homologue of MASP-2 and of rat 
      and mouse MAp19. We show that both MASP-2 and MAp19 are part of the rat MBL
      pathway activation complex and demonstrate their exclusively hepatic
      biosynthesis. Southern blot and PCR analyses of rat genomic DNA indicate that as 
      in humans, rat MASP-2 and MAp19 are encoded by a single structural gene.
FAU - Stover, C M
AU  - Stover CM
AD  - Department of Microbiology and Immunology, University of Leicester, United
      Kingdom.
FAU - Thiel, S
AU  - Thiel S
FAU - Lynch, N J
AU  - Lynch NJ
FAU - Schwaeble, W J
AU  - Schwaeble WJ
LA  - eng
SI  - GENBANK/AJ250369
SI  - GENBANK/Y18285
SI  - GENBANK/Y18564
SI  - GENBANK/Y18565
SI  - GENBANK/Y18566
SI  - GENBANK/Y18567
SI  - GENBANK/Y18568
SI  - GENBANK/Y18569
SI  - GENBANK/Y18570
SI  - GENBANK/Y18571
SI  - GENBANK/Y18572
SI  - GENBANK/Y18573
SI  - GENBANK/Y19160
SI  - GENBANK/Y19161
SI  - GENBANK/Y19162
SI  - GENBANK/Y19163
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Carrier Proteins)
RN  - 0 (Collectins)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (Lectins)
RN  - 0 (Mannans)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.21.- (MASP-2 protein, mouse)
RN  - EC 3.4.21.- (MASP2 protein, human)
RN  - EC 3.4.21.- (Mannose-Binding Protein-Associated Serine Proteases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Carrier Proteins/biosynthesis/blood/*chemistry/genetics
MH  - Cloning, Molecular
MH  - Collectins
MH  - *Complement Activation
MH  - DNA Probes
MH  - DNA, Complementary/analysis
MH  - Exons
MH  - Introns
MH  - Lectins/*immunology
MH  - Mannans/immunology
MH  - Mannose-Binding Protein-Associated Serine Proteases
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis/chemistry/isolation & purification
MH  - Rats
MH  - Rats, Wistar
MH  - *Sequence Homology, Amino Acid
MH  - Serine Endopeptidases/biosynthesis/blood/*chemistry/genetics
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - ji_v163n12p6848 [pii]
PST - ppublish
SO  - J Immunol. 1999 Dec 15;163(12):6848-59.