PMID- 10585886
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 344 Pt 3
DP  - 1999 Dec 15
TI  - Organization and sequence of the gene for the human mitochondrial dicarboxylate
      carrier: evolution of the carrier family.
PG  - 953-60
AB  - The dicarboxylate carrier (DIC) is a nuclear-encoded protein located in the
      mitochondrial inner membrane. It catalyses the transport of dicarboxylates such
      as malate and succinate across the mitochondrial membrane in exchange for
      phosphate, sulphate and thiosulphate. We have determined the sequences of the
      human cDNA and gene for the DIC. The gene sequence was established from
      overlapping genomic clones generated by PCRs by use of primers and probes based
      upon the human cDNA sequence. It is spread over 8.6 kb of human DNA and is
      divided into 11 exons. Five short interspersed repetitive Alu sequences are found
      in intron I. The protein encoded by the gene is 287 amino acids long. In common
      with the rat protein, it does not have a processed presequence to help to target 
      it into mitochondria. It has been demonstrated by Northern- and Western-blot
      analyses that the DIC is present in high amounts in liver and kidney, and at
      lower levels in all the other tissues analysed. The positions of introns
      contribute towards an understanding of the processes involved in the evolution of
      human genes for carrier proteins.
FAU - Fiermonte, G
AU  - Fiermonte G
AD  - Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology,
      University of Bari, Via E. Orabona 4, 70125 Bari, Italy.
FAU - Dolce, V
AU  - Dolce V
FAU - Arrigoni, R
AU  - Arrigoni R
FAU - Runswick, M J
AU  - Runswick MJ
FAU - Walker, J E
AU  - Walker JE
FAU - Palmieri, F
AU  - Palmieri F
LA  - eng
SI  - GENBANK/AJ131612
SI  - GENBANK/AJ131613
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Carrier Proteins)
RN  - 0 (Dicarboxylic Acid Transporters)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Alu Elements
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport/genetics
MH  - Carrier Proteins/*genetics
MH  - Cloning, Molecular
MH  - Dicarboxylic Acid Transporters
MH  - Evolution, Molecular
MH  - Exons
MH  - Humans
MH  - Introns
MH  - Kidney/metabolism
MH  - Liver/metabolism
MH  - Mitochondria/*metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Sequence Alignment
MH  - Sequence Analysis
PMC - PMC1220721
EDAT- 1999/12/10 09:00
MHDA- 2000/03/25 09:00
CRDT- 1999/12/10 09:00
PHST- 1999/12/10 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 1999/12/10 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Dec 15;344 Pt 3:953-60.