PMID- 10585884
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 344 Pt 3
DP  - 1999 Dec 15
TI  - Expression of glypican-4 in haematopoietic-progenitor and bone-marrow-stromal
      cells.
PG  - 937-43
AB  - Heparan sulphate proteoglycans and the extracellular matrix of
      bone-marrow-stromal cells are important components of the microenvironment of
      haematopoietic tissues and are involved in the interaction of haematopoietic stem
      and stromal cells. Previous studies have emphasized the role of heparan sulphate 
      proteoglycan synthesis by bone-marrow-stromal cells. In the present study we
      describe the expression of glypican-4 (GPC-4), belonging to the glypican family, 
      in bone-marrow-stromal cells and haematopoietic-progenitor cells of human and
      murine origin. Expression of GPC-4 was shown on the mRNA-level by reverse
      transcription-PCR and Northern blot analysis. Amplification products were cloned 
      and sequenced, to confirm these results. To analyze the expression of GPC-4 on
      the protein level, polyclonal antibodies against selected peptides were raised in
      rabbits. Western blot analysis showed expression of GPC-4 as a heparan sulphate
      proteoglycan in the human haematopoietic-progenitor cell line TF-1 and normal
      human bone marrow. These results were confirmed by FACS analysis of TF-1 cells.
      Furthermore, GPC-4-positive progenitor cells and stromal cells were enriched from
      normal human bone marrow by magnetic-cell sorting and analysed by confocal
      laser-scanning microscopy.
FAU - Siebertz, B
AU  - Siebertz B
AD  - Institute for Clinical Chemistry, University of Technology, Pauwelsstrasse 30,
      D-52057 Aachen, Germany.
FAU - Stocker, G
AU  - Stocker G
FAU - Drzeniek, Z
AU  - Drzeniek Z
FAU - Handt, S
AU  - Handt S
FAU - Just, U
AU  - Just U
FAU - Haubeck, H D
AU  - Haubeck HD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (GPC4 protein, human)
RN  - 0 (Glypicans)
RN  - 0 (Gpc4 protein, mouse)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow Cells/*metabolism
MH  - Cloning, Molecular
MH  - Flow Cytometry
MH  - Glypicans
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cells/*metabolism
MH  - Heparan Sulfate Proteoglycans/*genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stromal Cells/metabolism
PMC - PMC1220719
EDAT- 1999/12/10 09:00
MHDA- 2000/03/25 09:00
CRDT- 1999/12/10 09:00
PHST- 1999/12/10 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 1999/12/10 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Dec 15;344 Pt 3:937-43.