PMID- 10585854
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 344 Pt 3
DP  - 1999 Dec 15
TI  - Characterization of a novel transcript of prostaglandin endoperoxide H synthase 1
      with a tissue-specific profile of expression.
PG  - 677-85
AB  - The enzyme prostaglandin endoperoxide H synthase (PGHS) has a pivotal role in the
      prostanoid biosynthetic pathway because it catalyses the formation of
      prostaglandin H(2) (PGH(2)), the common precursor of prostanoids. Two PGHS
      isoforms have been reported, PGHS-1 and PGHS-2, which have 61% identity (at the
      amino acid level) and 73% similarity (at the nucleotide level) between the two
      human enzymes. Transcription of the PGHS-1 gene leads to the formation of two
      transcripts (2.8 and 5.1 kb); two transcripts of 2.8 and 4.5 kb are produced from
      the PGHS-2 gene. By Northern blot analysis with the entire coding region of human
      PGHS-1, 2.8 and 5.1 kb transcripts as well as a novel 4.5 kb transcript were
      detected in the human megakaryoblastic cell line MEG-01. We designed a strategy
      to characterize the 4.5 kb PGHS transcript. Probes specific for each PGHS-1 and
      PGHS-2 were designed on the basis of the 3' untranslated region (3' UTR), where
      no similarity is present. The 4.5 kb transcript was detected only with the
      PGHS-1-specific 3' UTR probes and not with the PGHS-2-specific 3' UTR probe. To
      investigate the origin of the 4.5 kb PGHS-1 transcript, the remaining 947 bp of
      the 5.1 kb PGHS-1 transcript was generated by 3' rapid amplification of cDNA ends
      (3' RACE) and sequenced. A non-canonical polyadenylation signal (AAGAAA) located 
      upstream of a potential cleavage site (CA) was found and could generate the 4.5
      kb PGHS-1 transcript. Analysis of the sequence also produced several possible
      G/U-rich elements downstream of the potential cleavage site. An RNA dot-blot with
      50 different human tissues was probed with the 4.5 and 5.1 kb PGHS-1-specific
      probes. A signal for the 4.5 kb PGHS-1 transcript was detected in the bladder and
      appendix. Signals of lower intensity were detected in the colon, bone marrow,
      small intestine, uterus, prostate, peripheral leucocyte, lymph node and stomach. 
      In conclusion, our results suggest that the cell line MEG-01, the bladder and the
      appendix contain a new PGHS-1 transcript of 4.5 kb that can be produced from the 
      PGHS-1 gene and we provide a better strategy for distinguishing PGHS-1
      transcripts from PGHS-2.
FAU - Plant, M H
AU  - Plant MH
AD  - Department of Biochemistry, University of Ottawa, 451 Smyth Road, Ottawa,
      Ontario, Canada K1H 8M5.
FAU - Laneuville, O
AU  - Laneuville O
LA  - eng
SI  - GENBANK/UNKNOWN
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Appendix/metabolism
MH  - Base Sequence
MH  - Blood Platelets/enzymology
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Molecular Sequence Data
MH  - Prostaglandin-Endoperoxide Synthases/*genetics
MH  - RNA, Messenger/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Time Factors
MH  - Urinary Bladder/metabolism
PMC - PMC1220689
EDAT- 1999/12/10 09:00
MHDA- 2000/03/25 09:00
CRDT- 1999/12/10 09:00
PHST- 1999/12/10 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 1999/12/10 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Dec 15;344 Pt 3:677-85.