PMID- 10585853
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 344 Pt 3
IP  - Pt 3
DP  - 1999 Dec 15
TI  - Gene structure of mouse BIT/SHPS-1.
PG  - 667-75
AB  - BIT/SHPS-1/SIRPalpha/P84 is a unique molecule with a high degree of homology with 
      immune antigen recognition molecules (immunoglobulin, T-cell receptor and MHC), 
      and is highly expressed in the brain. The extracellular region contains three 
      immunoglobulin-like domains (V-type, C1-type and C1-type), and the intracellular 
      region contains two signalling motifs that interact with SHP-2 protein tyrosine 
      phosphatase. BIT-coated plates support cell-substrate adhesion and neurite 
      extension of neurons, and BIT participates in neuronal signal transduction. 
      Diversity of the V-type domain sequences of human BIT has been reported. In the 
      present study we analysed the structure of the mouse BIT gene (Bit). The protein 
      coding region consists of eight exons corresponding to a signal peptide, a V-type 
      domain, a C1-type domain, a C1-type domain, a transmembrane region and three 
      parts of one cytoplasmic region. The two signalling motifs are encoded in one 
      exon. Four splicing forms of mouse BIT were revealed. We also found the sequence 
      diversity in three mouse strains, namely BALB/c, 129/Sv and C57BL/6. The 
      substitution patterns of amino acids and nucleotides indicate positive pressure 
      to alter the amino acids in the V-type domain in evolution. Immunoblot analyses 
      showed that mouse BIT and human BITalpha are predominantly expressed in the 
      brain. On the bases of these findings we discuss the possibility that BIT 
      contributes to the genetic individuality and diversity of the brain.
FAU - Sano, S
AU  - Sano S
AD  - Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 
      194-8511, Japan. ssano@libra.ls.m-kagaku.co.jp
FAU - Ohnishi, H
AU  - Ohnishi H
FAU - Kubota, M
AU  - Kubota M
LA  - eng
SI  - GENBANK/AB018194
SI  - GENBANK/AB023430
SI  - GENBANK/AB024500
SI  - GENBANK/AB024501
SI  - GENBANK/AB024502
SI  - GENBANK/AB024503
SI  - GENBANK/AB024504
SI  - GENBANK/AB024505
SI  - GENBANK/AB024506
SI  - GENBANK/AB024507
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Antigens, Differentiation)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigens, Differentiation
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - Brain/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Exons
MH  - Gene Expression Regulation
MH  - Humans
MH  - Introns
MH  - Membrane Glycoproteins/chemistry/*genetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - *Neural Cell Adhesion Molecule L1
MH  - Neural Cell Adhesion Molecules/chemistry/*genetics
MH  - *Receptors, Immunologic
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
PMC - PMC1220688
EDAT- 1999/12/10 09:00
MHDA- 2000/03/25 09:00
CRDT- 1999/12/10 09:00
PHST- 1999/12/10 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 1999/12/10 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Dec 15;344 Pt 3(Pt 3):667-75.