PMID- 10585779
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20191210
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 62
IP  - 1
DP  - 1999 Nov 15
TI  - Isolation, characterization, and mapping of a zinc finger gene, ZFP95, containing
      both a SCAN box and an alternatively spliced KRAB A domain.
PG  - 119-22
AB  - A new zinc finger gene of the Kruppel family was identified by screening a human 
      fetal cartilage cDNA library with degenerate oligonucleotides. Sequence analysis 
      indicates that ZFP95 contains 12 highly conserved zinc finger motifs at the
      C-terminus and a SCAN box as well as a KRAB A domain at the N-terminus of the
      protein. ZFP95 represents a member of a new subclass of Kruppel zinc finger
      proteins containing both a SCAN box and a KRAB domain. Sequence comparison
      revealed that ZFP95 is the human ortholog of murine Zfp95, which is
      differentially expressed during spermatogenesis. We demonstrate that ZFP95 is
      ubiquitously expressed in adult and fetal tissues with the strongest expression
      in testis. Two transcripts, 4. 2 and 4.6 kb, were detected in all tissues tested.
      In testis, a third transcript of 3.8 kb was present. RT-PCR analysis confirmed
      alternative splicing for the KRAB A domain and an upstream exon leading to three 
      transcripts of ZFP95 with and without this transcriptional repressor domain.
      Finally, we show that ZFP95 maps on human chromosome 7q22 between the markers
      D7S651 and WI-5853.
CI  - Copyright 1999 Academic Press.
FAU - Dreyer, S D
AU  - Dreyer SD
AD  - Children's Hospital, University of Mainz, Langenbeckstrasse 1, Mainz, D-55101,
      Germany.
FAU - Zheng, Q
AU  - Zheng Q
FAU - Zabel, B
AU  - Zabel B
FAU - Winterpacht, A
AU  - Winterpacht A
FAU - Lee, B
AU  - Lee B
LA  - eng
SI  - GENBANK/AF170025
GR  - AR44738/AR/NIAMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fetal Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (ZKSCAN5 protein, human)
RN  - 0 (Zkscan5 protein, mouse)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Carrier Proteins/biosynthesis/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins
MH  - Exons/genetics
MH  - Fetal Proteins/biosynthesis/genetics
MH  - Gene Expression Regulation, Developmental
MH  - *Genes
MH  - Humans
MH  - Kruppel-Like Transcription Factors
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Protein Structure, Tertiary
MH  - RNA Splicing
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Spermatogenesis/genetics
MH  - Testis/metabolism
MH  - Transcription, Genetic
MH  - Zinc Fingers/*genetics
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - 10.1006/geno.1999.5981 [doi]
AID - S0888-7543(99)95981-5 [pii]
PST - ppublish
SO  - Genomics. 1999 Nov 15;62(1):119-22. doi: 10.1006/geno.1999.5981.