PMID- 10585770
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20060406
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 62
IP  - 1
DP  - 1999 Nov 15
TI  - Multiple MSP pseudogenes in a local repeat cluster on 1p36.2: An expanding
      genomic graveyard?
PG  - 74-81
AB  - Chromosomal region 1p36.2 harbors an intriguing gene cluster of about 1 Mb. In
      addition to normal high-copy-number repeats, this cluster consists entirely of
      locally repeated sequences among which there are tRNA and small nuclear RNA
      (snRNA) genes. In 23 PACs and YACs from the 1p36.2 cluster, we identified eight
      different copies of a sequence with about 97% homology to the macrophage
      stimulating protein (MSP) gene located on chromosomal band 3p21. These MSP-like
      (MSPL) sequences on 1p36.2 are scattered over the repeat region. Nucleotide
      substitutions and single nucleotide deletions in exons of all identified MSPL
      genes on 1p36.2 mark them as pseudogenes. We constructed a phylogenetic tree of
      these sequences with their most likely order of origin in evolution. MSP from
      3p21 could be identified as the ancestral sequence, a copy of which was captured 
      into the cluster of tRNA and snRNA genes on 1p36.2 about 6 million years (MY)
      ago. MSP subsequently coamplified with the other sequences in the cluster.
      Analysis of the DNA of 18 individuals shows that the MSPL copy number is
      polymorphic, with a range of four to seven or more copies per haploid genome.
      Analysis of corresponding clusters in macaque chromosomes indicated an age for
      the tRNA/snRNA cluster of at least 30 MY. The MSPL sequence thus functions as a
      probe for the more recent primate evolution of this cluster and suggests a
      continuation of its unusual activity over the last 6 MY.
CI  - Copyright 1999 Academic Press.
FAU - van der Drift, P
AU  - van der Drift P
AD  - Department of Human Genetics, Academic Medical Center, Amsterdam, The
      Netherlands.
FAU - Chan, A
AU  - Chan A
FAU - Zehetner, G
AU  - Zehetner G
FAU - Westerveld, A
AU  - Westerveld A
FAU - Versteeg, R
AU  - Versteeg R
LA  - eng
SI  - GENBANK/AF083410
SI  - GENBANK/AF083411
SI  - GENBANK/AF083412
SI  - GENBANK/AF083413
SI  - GENBANK/AF083414
SI  - GENBANK/AF083415
SI  - GENBANK/AF083416
SI  - GENBANK/AF083417
PT  - Journal Article
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Growth Substances)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (macrophage stimulating protein)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9014-25-9 (RNA, Transfer)
SB  - IM
MH  - Animals
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - *Evolution, Molecular
MH  - Gene Duplication
MH  - Growth Substances/*genetics
MH  - *Hepatocyte Growth Factor
MH  - Humans
MH  - Phylogeny
MH  - Primates/*genetics
MH  - *Proto-Oncogene Proteins
MH  - *Pseudogenes
MH  - RNA, Small Nuclear/genetics
MH  - RNA, Transfer/genetics
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - 10.1006/geno.1999.5972 [doi]
AID - S0888-7543(99)95972-4 [pii]
PST - ppublish
SO  - Genomics. 1999 Nov 15;62(1):74-81. doi: 10.1006/geno.1999.5972.