PMID- 10585767
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20161124
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 62
IP  - 1
DP  - 1999 Nov 15
TI  - Structure and expression of the gene encoding mouse F-box protein, Fwd2.
PG  - 50-8
AB  - A novel class of ubiquitin ligases, termed the SCF complex, consists of
      invariable components, Skp1 and Cullin, and variable components called F-box
      proteins, which have a primary role in determining substrate specificity. We have
      isolated a cDNA encoding the mouse F-box protein Fwd2 (also known as MD6) as a
      possible constituent of an SCF-type ubiquitin ligase. Fwd2 cDNA contains 1890 bp 
      with a 1362-bp open reading frame and encodes an approximately 51.5-kDa protein. 
      Fwd2 is expressed predominantly in liver and, to a lesser extent, in the testis, 
      lung, heart, and skeletal muscle. Immunofluorescence staining for Fwd2 protein
      shows a pattern with the cytoplasm. A coimmunoprecipitation assay has revealed
      the in vivo interaction between Skp1 and Fwd2 through the F-box domain. Fwd2 also
      interacts with Cul1 through Skp1, suggesting that Skp1, Cul1, and the F-box
      protein Fwd2 form an SCF complex (SCF(Fwd2)). We have also isolated and
      determined the nucleotide sequence and genomic organization of the gene that
      encodes mouse Fwd2. This gene spans approximately 17 kb and consists of six exons
      and five introns. Our results suggest that Fwd2 is an F-box protein that
      constitutes an SCF ubiquitin ligase complex and that it plays a critical role in 
      the ubiquitin-dependent degradation of proteins expressed in the liver.
CI  - Copyright 1999 Academic Press.
FAU - Miura, M
AU  - Miura M
AD  - Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Fukuoka,
      812-8582, Japan.
FAU - Hatakeyama, S
AU  - Hatakeyama S
FAU - Hattori, K
AU  - Hattori K
FAU - Nakayama, K
AU  - Nakayama K
LA  - eng
SI  - GENBANK/AF140683
SI  - GENBANK/AF140684
SI  - GENBANK/AF140685
SI  - GENBANK/AF140686
SI  - GENBANK/AF140687
SI  - GENBANK/AF140688
SI  - GENBANK/AF140689
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (F-Box Proteins)
RN  - 0 (Fbxw2 protein, mouse)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytoplasm/enzymology
MH  - DNA, Complementary/genetics
MH  - Exons/genetics
MH  - Expressed Sequence Tags
MH  - *F-Box Proteins
MH  - *Gene Expression Regulation
MH  - *Genes
MH  - Introns/genetics
MH  - Liver/enzymology
MH  - Mice/*genetics
MH  - Molecular Sequence Data
MH  - Peptide Synthases/chemistry/*genetics
MH  - Protein Structure, Tertiary
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - SKP Cullin F-Box Protein Ligases
MH  - Transfection
MH  - Ubiquitins/metabolism
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - 10.1006/geno.1999.5965 [doi]
AID - S0888754399959657 [pii]
PST - ppublish
SO  - Genomics. 1999 Nov 15;62(1):50-8. doi: 10.1006/geno.1999.5965.