PMID- 10585558
OWN - NLM
STAT- MEDLINE
DCOM- 20000228
LR  - 20190910
IS  - 0925-4773 (Print)
IS  - 0925-4773 (Linking)
VI  - 90
IP  - 1
DP  - 2000 Jan
TI  - Gene trapping of two novel genes, Hzf and Hhl, expressed in hematopoietic cells.
PG  - 3-15
AB  - Using an expression gene trapping strategy, we have identified and characterized 
      two novel hematopoietic genes, Hzf and Hhl. Embryonic stem (ES) cells containing 
      a gene trap vector insertion were cultured on OP9 stromal cells to induce
      hematopoietic differentiation and screened for lacZ reporter gene expression. Two
      ES clones displaying lacZ expression within hematopoietic cells in vitro were
      used to generate mice containing the gene trap integrations. Paralleling this in 
      vitro expression pattern, both Hzf and Hhl were expressed in a tissue-specific
      manner during hematopoietic development in vivo. Hzf encodes a novel protein
      containing three C(2)H(2)-type zinc fingers predominantly expressed in
      megakaryocytes and CFU-GEMM. Hhl encodes a novel protein containing a putative
      phosphotyrosine binding (PTB) domain expressed in megakaryocytes, CFU-GEMM and
      BFU-E. These results demonstrate the utility of expression trapping to identify
      novel hematopoietic genes. Future studies of Hzf and Hhl should provide valuable 
      information on the role these genes play during megakaryocytopoiesis.
FAU - Hidaka, M
AU  - Hidaka M
AD  - Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute,
      Mount Sinai Hospital, 600 University Avenue, Toronto, Canada.
FAU - Caruana, G
AU  - Caruana G
FAU - Stanford, W L
AU  - Stanford WL
FAU - Sam, M
AU  - Sam M
FAU - Correll, P H
AU  - Correll PH
FAU - Bernstein, A
AU  - Bernstein A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mech Dev
JT  - Mechanisms of development
JID - 9101218
RN  - 0 (Blood Proteins)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Proteins)
RN  - 0 (Rabgap1l protein, mouse)
RN  - 0 (Zfp385 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blood Proteins/*genetics
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - GTPase-Activating Proteins
MH  - *Gene Expression Regulation, Developmental
MH  - Hematopoiesis/*genetics
MH  - Hematopoietic Stem Cells/cytology/*physiology
MH  - In Situ Hybridization
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - *Proteins
MH  - Stem Cells/cytology/*physiology
MH  - Stromal Cells/cytology/*physiology
EDAT- 1999/12/10 09:00
MHDA- 2000/03/04 09:00
CRDT- 1999/12/10 09:00
PHST- 1999/12/10 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/12/10 09:00 [entrez]
AID - S0925-4773(99)00234-8 [pii]
AID - 10.1016/s0925-4773(99)00234-8 [doi]
PST - ppublish
SO  - Mech Dev. 2000 Jan;90(1):3-15. doi: 10.1016/s0925-4773(99)00234-8.