PMID- 10585455
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 50
DP  - 1999 Dec 10
TI  - Molecular cloning of six novel Kruppel-like zinc finger genes from hematopoietic 
      cells and identification of a novel transregulatory domain KRNB.
PG  - 35741-8
AB  - To clone zinc finger genes expressed in hematopoietic system, we designed primers
      based on conserved Cys(2)/His(2) zinc finger sequences to amplify corresponding
      domains from mRNA of normal bone marrow and leukemia cell line NB4. DNA fragments
      of novel zinc finger genes were chosen and used as probe pool to screen cDNA
      libraries or subject to rapid amplification of cDNA ends in order to obtain
      full-length cDNA. Six cDNAs including whole open reading frame of zinc finger
      proteins, named as ZNF191, ZNF253 (BMZF-1), ZNF255 (BMZF-2), ZNF256 (BMZF-3),
      ZNF257 (BMZF-4), and ZNF254 (BMZF-5) were obtained. All six belong to the
      Kruppel-like zinc finger gene family, and typical transcriptional regulatory
      motifs exist in the N-terminal moiety, such as the SCAN box in ZNF191, and the
      KRAB domains in ZNF253, ZNF254, ZNF256, and ZNF257. A previously undefined
      sequence nominated as Kruppel-related novel box, which may represent a new
      transregulatory motif, was revealed at the N terminus of ZNF255. The
      transregulatory function of non-zinc finger regions of ZNF191, ZNF253, and ZNF255
      were addressed in yeast and mammalian cells. The results indicated that ZNF255
      might be a conditional transactivator, whereas ZNF253 and ZNF191 displayed a
      suppressive effect on the transcription in yeast and/or mammalian systems.
FAU - Han, Z G
AU  - Han ZG
AD  - Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, 201203 
      Shanghai, China. zchen@ms.stn.sh.cn
FAU - Zhang, Q H
AU  - Zhang QH
FAU - Ye, M
AU  - Ye M
FAU - Kan, L X
AU  - Kan LX
FAU - Gu, B W
AU  - Gu BW
FAU - He, K L
AU  - He KL
FAU - Shi, S L
AU  - Shi SL
FAU - Zhou, J
AU  - Zhou J
FAU - Fu, G
AU  - Fu G
FAU - Mao, M
AU  - Mao M
FAU - Chen, S J
AU  - Chen SJ
FAU - Yu, L
AU  - Yu L
FAU - Chen, Z
AU  - Chen Z
LA  - eng
SI  - GENBANK/AF038951
SI  - GENBANK/AF038964
SI  - GENBANK/AF054180
SI  - GENBANK/AF067164
SI  - GENBANK/AF067165
SI  - GENBANK/AF070651
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bone Marrow Cells/cytology/metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Evolution, Molecular
MH  - Fetal Blood
MH  - Hematopoietic Stem Cells/cytology/*metabolism
MH  - Humans
MH  - Leukemia, Promyelocytic, Acute
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Organ Specificity
MH  - Phylogeny
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
MH  - Zinc Fingers/*genetics
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - 10.1074/jbc.274.50.35741 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 10;274(50):35741-8. doi: 10.1074/jbc.274.50.35741.