PMID- 10585392
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 50
DP  - 1999 Dec 10
TI  - COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
PG  - 35297-300
AB  - The basic region-leucine zipper transcription factor c-Jun regulates gene
      expression and cell function. It participates in the formation of homo- or
      heterodimeric complexes that specifically bind to DNA sequences called activating
      protein 1 (AP-1) sites. The stability and activity of c-Jun is regulated by
      phosphorylation within the N-terminal activation domain. Mitogen- and
      stress-activated c-Jun N-terminal kinases (JNKs) were previously the only
      described enzymes phosphorylating c-Jun at the N terminus in vivo. In this report
      we demonstrate a JNK-independent activation of c-Jun in vivo directed by the
      constitutive photomorphogenesis (COP9) signalosome. The overexpression of
      signalosome subunit 2 (Sgn2), a subunit of the COP9 signalosome, leads to de novo
      assembly of the complex with a partial incorporation of the overexpressed
      subunit. The de novo formation of COP9 signalosome parallels an increase of c-Jun
      protein resulting in elevated AP-1 transcriptional activity. The c-Jun activation
      caused by Sgn2 overexpression is independent of JNK and mitogen-activated protein
      kinase kinase 4. The data demonstrate the existence of a novel COP9
      signalosome-directed c-Jun activation pathway.
FAU - Naumann, M
AU  - Naumann M
AD  - Max-Planck-Institut fur Infektionsbiologie, Abteilung Molekulare Biologie,
      Humboldt University, 10117 Berlin, Germany. naumann@mpiib-berlin.mpg.de
FAU - Bech-Otschir, D
AU  - Bech-Otschir D
FAU - Huang, X
AU  - Huang X
FAU - Ferrell, K
AU  - Ferrell K
FAU - Dubiel, W
AU  - Dubiel W
LA  - eng
SI  - GENBANK/AF084260
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (COPS2 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GPS1 protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (MAP2K4 protein, human)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.-.- (COPS5 protein, human)
RN  - EC 3.4.19.12 (COP9 Signalosome Complex)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - COP9 Signalosome Complex
MH  - DNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Erythrocytes/metabolism
MH  - *GTP-Binding Proteins
MH  - Genes, Reporter
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - JNK Mitogen-Activated Protein Kinases
MH  - *MAP Kinase Kinase 4
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Molecular Sequence Data
MH  - Peptide Hydrolases
MH  - Phosphorylation
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Proteins/genetics/isolation & purification/*metabolism
MH  - Proto-Oncogene Proteins c-jun/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Recombinant Proteins/metabolism
MH  - *Repressor Proteins
MH  - Sequence Deletion
MH  - Transcription Factor AP-1/metabolism
MH  - Transcription Factors/genetics/isolation & purification/*metabolism
MH  - *Transcriptional Activation
MH  - Transfection
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - 10.1074/jbc.274.50.35297 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 10;274(50):35297-300. doi: 10.1074/jbc.274.50.35297.