PMID- 10585290
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20061115
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 253
IP  - 2
DP  - 1999 Dec 15
TI  - Domain analysis of the actin-binding and actin-remodeling activities of drebrin.
PG  - 673-80
AB  - Drebrin is an actin-binding protein which is expressed at highly levels in
      neurons. When introduced into fibroblasts, it has been known to bind to F-actin
      and to cause remodeling of F-actin. Here, we performed a domain analysis of the
      actin-binding and actin-remodeling activities of drebrin. Various fragments of
      drebrin cDNA were fused with green fluorescent protein cDNA and introduced into
      Chinese hamster ovary cells. Association of the fusion protein with F-actin and
      remodeling of the F-actin were examined. We found that the central 85-amino-acid 
      sequence (residues 233-317) was sufficient for the binding to and remodeling of
      F-actin. The binding activity of this fragment was relatively low compared with
      that of full-length drebrin, but all the types of abnormalities of F-actin that
      are observed with full-length drebrin were also observed with this fragment. When
      this sequence was further fragmented, the actin-binding activity was greatly
      reduced and the actin-remodeling activity disappeared. The actin-binding activity
      of the central region of drebrin was confirmed by a cosedimentation assay of
      chymotryptic fragments of drebrin with purified actin. These data indicate that
      the actin-binding domain and actin-remodeling domain are identical and that this 
      domain is located at the central region of drebrin.
CI  - Copyright 1999 Academic Press.
FAU - Hayashi, K
AU  - Hayashi K
AD  - Department of Neurobiology and Behavior, Department of Pharmacology, Gunma
      University School of Medicine, 3-39-22 Showamachi, Maebashi, 371, Japan.
      hayashi@sb.gunma-u.ac.jp
FAU - Ishikawa, R
AU  - Ishikawa R
FAU - Kawai-Hirai, R
AU  - Kawai-Hirai R
FAU - Takagi, T
AU  - Takagi T
FAU - Taketomi, A
AU  - Taketomi A
FAU - Shirao, T
AU  - Shirao T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Actins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (drebrins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.4.21.1 (Chymotrypsin)
SB  - IM
MH  - Actins/*metabolism
MH  - Animals
MH  - Blotting, Western
MH  - CHO Cells
MH  - Chymotrypsin
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins
MH  - Indicators and Reagents/metabolism
MH  - Luminescent Proteins/genetics
MH  - Microfilament Proteins/*chemistry/genetics/*metabolism
MH  - Mutagenesis, Insertional/physiology
MH  - Neuropeptides/*chemistry/genetics/*metabolism
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Phosphorylation
MH  - Protein Binding/physiology
MH  - Recombinant Fusion Proteins/analysis
MH  - Stress, Mechanical
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - 10.1006/excr.1999.4663 [doi]
AID - S0014-4827(99)94663-3 [pii]
PST - ppublish
SO  - Exp Cell Res. 1999 Dec 15;253(2):673-80. doi: 10.1006/excr.1999.4663.