PMID- 10585123
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20190921
IS  - 0919-8172 (Print)
IS  - 0919-8172 (Linking)
VI  - 6
IP  - 11
DP  - 1999 Nov
TI  - Expression of protein tyrosine phosphatase PTP-RL10 and its isoform in the mouse 
      testis.
PG  - 572-7
AB  - BACKGROUND: The cytoplasmic-type protein tyrosine phosphatase
      PTP-RL10/PTPD1/PTP2E contains an ezrin-like domain and associates with the c-Src 
      protein tyrosine kinase. Because tyrosine phosphorylation regulated by protein
      tyrosine kinases and phosphatases is involved in activation, migration,
      differentiation and proliferation of various cell types, the expression of
      PTP-RL10 and c-src in the mouse testis was investigated. METHODS: Testes of
      wild-type mice and W/W(v) mutant mice that lack germ cells were analyzed by
      northern blotting, in situ hybridization histochemistry and reverse
      transcriptase-polymerase chain reaction for the expression of PTP-RL10, its
      isoform PTP-RL10b and c-src. Expression in the Sertoli cell lines, TM4 and TAMA26
      was also analyzed. RESULTS: PTP-RL10 transcripts of 5.7kb and 2.9kb in size were 
      detected in the testis. In situ hybridization histochemistry demonstrated that
      the 5.7kb transcripts were expressed in pachytene spermatocytes and somatic cells
      including Sertoli cells, in which c-src transcripts were detected. The 2.9kb
      transcript encoded a novel isoform, PTP-RL10b, that has the catalytic domain but 
      not the domains that associate with c-Src. PTP-RL10b was expressed mainly in the 
      testicular somatic cells. TM4 and TAMA26 cells were found to co-express PTP-RL10,
      PTP-RL10b and c-src transcripts. CONCLUSION: PTP-RL10 and its isoform are
      expressed in the Sertoli cells and are suggested to play roles in spermatogenesis
      by interacting with c-Src and/or other protein(s).
FAU - Tokuchi, H
AU  - Tokuchi H
AD  - Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 
      Japan.
FAU - Higashitsuji, H
AU  - Higashitsuji H
FAU - Nishiyama, H
AU  - Nishiyama H
FAU - Nonoguchi, K
AU  - Nonoguchi K
FAU - Nagao, T
AU  - Nagao T
FAU - Xue, J H
AU  - Xue JH
FAU - Itoh, K
AU  - Itoh K
FAU - Ogawa, O
AU  - Ogawa O
FAU - Fujita, J
AU  - Fujita J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Int J Urol
JT  - International journal of urology : official journal of the Japanese Urological
      Association
JID - 9440237
RN  - 0 (Protein Isoforms)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Gene Expression
MH  - Germ Cells
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Protein Isoforms
MH  - Protein Tyrosine Phosphatases/*analysis
MH  - Sertoli Cells
MH  - Spermatogenesis
MH  - Testis/*enzymology
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - 10.1046/j.1442-2042.1999.611108.x [doi]
PST - ppublish
SO  - Int J Urol. 1999 Nov;6(11):572-7. doi: 10.1046/j.1442-2042.1999.611108.x.