PMID- 10583409
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 266
IP  - 3
DP  - 1999 Dec
TI  - Striatum- and testis-specific phosphodiesterase PDE10A isolation and
      characterization of a rat PDE10A.
PG  - 1118-27
AB  - PDE10A, a phosphodiesterase (PDE) exhibiting properties of a cAMP PDE and a
      cAMP-inhibited cGMP PDE, was cloned and investigated in detail in rats. PDE10A
      transcripts were abundant in the brain and testis. In situ hybridization analysis
      using a PDE10A riboprobe demonstrated the presence of PDE10A transcripts in the
      neurons of the striatum and the olfactory tubercle regions of the brain. Rat
      PDE10A cDNAs were isolated from a brain cDNA library and nucleotide sequence
      analysis revealed several N-terminal variants. The deduced amino-acid sequence of
      one of the major variant forms contained 794 amino acids, and it was 96%
      identical to that of the human PDE10A2. The other major form has a distinct
      N-terminal sequence that is not found in humans. PDE10A was partially purified
      from rat striatum and testis, and characterized with respect to Km, inhibitor
      sensitivity and immunoreactivity to an anti-PDE10A serum. These findings indicate
      that PDE10A functions in these tissues.
FAU - Fujishige, K
AU  - Fujishige K
AD  - Discovery Research Laboratory, Tanabe Seiyaku Co. Ltd. Saitama, Japan.
FAU - Kotera, J
AU  - Kotera J
FAU - Omori, K
AU  - Omori K
LA  - eng
SI  - GENBANK/AB027155
SI  - GENBANK/AB027156
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.1.4.- (PDE10A protein, human)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Chromatography, Ion Exchange
MH  - Cloning, Molecular
MH  - Corpus Striatum/*enzymology
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Genetic Variation
MH  - Humans
MH  - In Situ Hybridization
MH  - Kinetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Phosphoric Diester Hydrolases/*genetics/*isolation & purification/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Recombinant Proteins/genetics/immunology
MH  - Sequence Homology, Amino Acid
MH  - Testis/*enzymology
MH  - Tissue Distribution
MH  - Transfection
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - ejb963 [pii]
AID - 10.1046/j.1432-1327.1999.00963.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Dec;266(3):1118-27. doi: 10.1046/j.1432-1327.1999.00963.x.