PMID- 10583383
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 266
IP  - 3
DP  - 1999 Dec
TI  - Enovin, a member of the glial cell-line-derived neurotrophic factor (GDNF) family
      with growth promoting activity on neuronal cells. Existence and tissue-specific
      expression of different splice variants.
PG  - 892-902
AB  - Glial cell-line-derived neurotrophic factor (GDNF), neurturin and persephin are
      neurotrophic factors involved in neuroneal differentiation, development and
      maintenance. They act on different types of neuroneal cells and signal through a 
      receptor complex composed of a specific ligand-binding subunit of the GDNF family
      receptor alpha (GFRalpha) family together with a common signaling partner, the
      cRET protein tyrosine kinase. We describe the molecular cloning, expression,
      chromosomal localization and functional characterization of enovin, a fourth GDNF
      family member almost identical to the recently described artemin. We show the
      occurence in most tissues of several differently spliced mRNA variants for
      enovin, of which only two are able to translate into functional enovin protein.
      Some tissues seem to express only nonfunctional transcripts. These observations
      may underlie a complex transcriptional regulation pattern. Enovin mRNA expression
      is detectable in all adult and fetal human tissues examined, but expression
      levels are highest in peripheral tissues including prostate, placenta, pancreas, 
      heart and kidney. This tissue distribution pattern is in accordance with that of 
      GFRalpha-3, which here is shown to be the preferred ligand-binding receptor for
      enovin (Kd = 3.1 nM). The human enovin gene is localized on chromosome 1, region 
      p31.3-p32. In vitro, enovin stimulates neurite outgrowth and counteracts
      taxol-induced neurotoxicity in staurosporine-differentiated SH-SY5Y human
      neuroblastoma cells. The peripheral expression pattern of enovin and its receptor
      together with its effects on neuroneal cells suggest that enovin might be useful 
      for the treatment of neurodegenerative diseases in general and peripheral
      neuropathies in particular.
FAU - Masure, S
AU  - Masure S
AD  - Department of Biotechnology & High-Throughput Screening, Janssen Research
      Foundation, Beerse, Belium. smasure@janbe.jnj.com
FAU - Geerts, H
AU  - Geerts H
FAU - Cik, M
AU  - Cik M
FAU - Hoefnagel, E
AU  - Hoefnagel E
FAU - Van Den Kieboom, G
AU  - Van Den Kieboom G
FAU - Tuytelaars, A
AU  - Tuytelaars A
FAU - Harris, S
AU  - Harris S
FAU - Lesage, A S
AU  - Lesage AS
FAU - Leysen, J E
AU  - Leysen JE
FAU - Van Der Helm, L
AU  - Van Der Helm L
FAU - Verhasselt, P
AU  - Verhasselt P
FAU - Yon, J
AU  - Yon J
FAU - Gordon, R D
AU  - Gordon RD
LA  - eng
SI  - GENBANK/AJ245628
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (GDNF protein, human)
RN  - 0 (GFRA3 protein, human)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Proteins)
RN  - ST1KS15P0T (enovin)
SB  - IM
MH  - Adult
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Escherichia coli/genetics
MH  - Female
MH  - Gene Expression
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Glial Cell Line-Derived Neurotrophic Factor Receptors
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*genetics/pharmacology/*physiology
MH  - Nerve Tissue Proteins/*genetics/pharmacology/*physiology
MH  - Neurons/cytology/drug effects/physiology
MH  - Pregnancy
MH  - Recombinant Proteins/genetics/pharmacology
MH  - Tissue Distribution
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - ejb925 [pii]
AID - 10.1046/j.1432-1327.1999.00925.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Dec;266(3):892-902. doi: 10.1046/j.1432-1327.1999.00925.x.