PMID- 10583376
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 266
IP  - 3
DP  - 1999 Dec
TI  - Solution structure of a new hypothalamic neuropeptide, human
      hypocretin-2/orexin-B.
PG  - 831-9
AB  - Orexin-A and orexin-B (also called hypocretin-1 and hypocretin-2, respectively)
      are novel hypothalamic neuropeptides encoded by a single mRNA transcript; they
      stimulate food intake. We have determined the three-dimensional solution
      structure of human hypocretin-2/orexin-B using two-dimensional 1H-NMR data and
      dynamical simulated annealing calculations. On the basis of NOEs, 3JHNalpha
      coupling constants and hydrogen-deuterium exchange rates together with chemical
      shift indices, human hypocretin-2/orexin-B was deduced to consist of two
      alpha-helices connected with a short linker in both H2O and 30% trifluoroethanol 
      solutions. The helical axis of helix I is oriented about 60-80 degrees relative
      to helix II. Hybrid distance geometry and simulated-annealing protocols were used
      to generate an ensemble of 30 structures with no constraint violations greater
      than 0.03 nm for distances and 3 degrees for angles. In addition, human
      hypocretin-2/orexin-B shares a similar secondary-structural motif with human
      neuropeptide Y. This result can form the basis for further study on
      ligand-receptor recognition of human orexin receptors.
FAU - Lee, J H
AU  - Lee JH
AD  - Department of Biochemistry College of Science, Yonsei University, Seoul, Korea.
FAU - Bang, E
AU  - Bang E
FAU - Chae, K J
AU  - Chae KJ
FAU - Kim, J Y
AU  - Kim JY
FAU - Lee, D W
AU  - Lee DW
FAU - Lee, W
AU  - Lee W
LA  - eng
SI  - PDB/1CQO
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (HCRT protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Neuropeptides)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Orexins)
RN  - 0 (Solutions)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Circular Dichroism
MH  - Humans
MH  - Hypothalamus/*chemistry
MH  - Intracellular Signaling Peptides and Proteins
MH  - Magnetic Resonance Spectroscopy
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Neuropeptide Y/chemistry
MH  - Neuropeptides/*chemistry/genetics
MH  - Neurotransmitter Agents/*chemistry/genetics
MH  - Obesity/etiology/metabolism
MH  - Orexins
MH  - Protein Structure, Secondary
MH  - Sequence Homology, Amino Acid
MH  - Solutions
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - ejb911 [pii]
AID - 10.1046/j.1432-1327.1999.00911.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Dec;266(3):831-9. doi: 10.1046/j.1432-1327.1999.00911.x.