PMID- 10582615
OWN - NLM
STAT- MEDLINE
DCOM- 19991227
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 6
DP  - 1999 Dec
TI  - A novel microtubule-associated protein-2 expressed in oligodendrocytes in
      multiple sclerosis lesions.
PG  - 2531-7
AB  - Elucidation of the mechanisms involved in the regeneration of oligodendrocytes
      and remyelination is a central issue in multiple sclerosis (MS) research. We
      recently identified a novel alternatively spliced, developmentally regulated
      oligodendrocyte-specific protein designated microtubule-associated protein-2+13
      [microtubule-associated protein-2 expressing exon 13 (MAP-2+13)]. MAP-2+13 is
      expressed in human fetal oligodendrocytes during process extension and
      myelination but is minimally expressed in normal mature CNS. To test the
      hypothesis that MAP-2+13 is reexpressed in regenerating oligodendrocytes in MS
      lesions, we examined the brains of MS patients for the expression of this
      protein. By immunocytochemistry using a series of monoclonal antibodies specific 
      for MAP-2+13, we determined that MAP-2+13 expression was up-regulated in all 31
      lesions from 10 different MS brains. MAP-2+13 was expressed in regenerating
      oligodendrocytes associated with demyelinated lesions, with the highest counts
      found in regions of extensive remyelination. By electron microscopy, MAP-2+13 was
      localized to oligodendrocytes engaged in remyelination, evident by their process 
      extension and association with thinly myelinated (remyelinated) and demyelinated 
      axons. These results suggest a hitherto unsuspected role for this
      microtubule-associated protein in oligodendrocyte function during development and
      myelin repair.
FAU - Shafit-Zagardo, B
AU  - Shafit-Zagardo B
AD  - Department of Pathology, Albert Einstein College of Medicine, Bronx, New York
      10461, USA.
FAU - Kress, Y
AU  - Kress Y
FAU - Zhao, M L
AU  - Zhao ML
FAU - Lee, S C
AU  - Lee SC
LA  - eng
GR  - NS38102/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Axons/pathology
MH  - Exons/*genetics
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Microscopy, Electron
MH  - Microtubule-Associated Proteins/biosynthesis/genetics/*isolation & purification
MH  - Microtubules/physiology/ultrastructure
MH  - Middle Aged
MH  - Multiple Sclerosis/*metabolism/pathology
MH  - Myelin Sheath/physiology
MH  - Nerve Tissue Proteins/*metabolism
MH  - Oligodendroglia/*metabolism
MH  - Paraparesis, Spastic/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0732531.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Dec;73(6):2531-7. doi: 10.1046/j.1471-4159.1999.0732531.x.