PMID- 10582586
OWN - NLM
STAT- MEDLINE
DCOM- 19991227
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 6
DP  - 1999 Dec
TI  - CTCF is essential for up-regulating expression from the amyloid precursor protein
      promoter during differentiation of primary hippocampal neurons.
PG  - 2286-98
AB  - The transcriptional mechanism underlying amyloid precursor protein (APP)
      regulation in primary neurons during development was investigated. We observed an
      approximately threefold elevation of APP mRNA levels in differentiating rat
      hippocampal neurons between day 1 and day 7 in culture and in rat brain
      hippocampi between embryonic day 18 and postnatal day 3. When an APP promoter
      construct extending to position -2,832 upstream from the main transcriptional
      start site was transfected into primary rat hippocampal neurons, promoter
      activity increased from day 1 until reaching a maximum on day 7 in culture. This 
      increase in APP promoter activity was correlated more closely with the time
      course of expression of the synaptic vesicle protein synaptophysin, an indicator 
      of synaptogenesis, than with neurofilament accumulation, an indicator of
      neuritogenesis. Transfection of 5' APP promoter deletions and internal block
      mutations indicated that the CTCF binding domain designated APBbeta was the
      primary contributor to the increase in APP promoter activity. Furthermore, the
      binding of transcription factor CTCF to the APBbeta element increased
      approximately fivefold between day 1 and day 7, whereas the binding of USF to the
      APBalpha sequence increased only twofold. These results suggest that CTCF is
      pivotal for the up-regulation of APP expression during synaptogenesis in primary 
      neurons.
FAU - Yang, Y
AU  - Yang Y
AD  - Department of Medical Microbiology and Immunology, Southern Illinois University
      School of Medicine, Springfield 62794-9626, USA.
FAU - Quitschke, W W
AU  - Quitschke WW
FAU - Vostrov, A A
AU  - Vostrov AA
FAU - Brewer, G J
AU  - Brewer GJ
LA  - eng
GR  - AG13435/AG/NIA NIH HHS/United States
GR  - NS30994/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (CCCTC-Binding Factor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Synaptophysin)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Amyloid beta-Protein Precursor/*biosynthesis/genetics
MH  - Animals
MH  - Base Sequence
MH  - CCCTC-Binding Factor
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/*physiology
MH  - Gene Expression Regulation/*physiology
MH  - Hippocampus/*cytology
MH  - Molecular Sequence Data
MH  - Neurofilament Proteins/metabolism
MH  - Neurons/*cytology/metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Recombinant Fusion Proteins/metabolism
MH  - *Repressor Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synaptophysin/biosynthesis
MH  - Transcription Factors/*physiology
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0732286.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Dec;73(6):2286-98. doi: 10.1046/j.1471-4159.1999.0732286.x.