PMID- 10581401
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 73
IP  - 1-2
DP  - 1999 Nov 10
TI  - Evidence for alternative splicing of ecto-ATPase associated with termination of
      purinergic transmission.
PG  - 85-92
AB  - Ectonucleotidases provide the signal termination mechanism for purinergic
      transmission, including fast excitatory neurotransmission by ATP in the CNS. This
      study provides evidence for ectonucleotidase expression in the rat cochlea, brain
      and other tissues. In addition to detection of rat ecto-ATPase and ecto-ATPDase
      in these tissues, we identify a novel ecto-ATPase splice variant arising from the
      loss of a putative exon (193 bp) in the C-terminal coding region. This is the
      first evidence of alternative splicing in the ecto-ATPase gene family. Splicing
      of the 193-bp putative exon containing a stop codon extends the open reading
      frame and provides translation of an additional 50 amino acids compared with the 
      isoform isolated earlier from the rat brain (rEATPase(A); GenBank accession
      #Y11835). The splice variant (rEATPase(B); GenBank accession #AF129103) encodes
      545 amino acids with a predicted protein molecular mass of 60 kDa. rEATPase(B)
      contains a long cytoplasmic tail (62 amino acids) with three potential protein
      kinase CK2 phosphorylation sites not present in rEATPase(A). Co-expression of two
      ecto-ATPase isoforms with different regulatory sites suggests that the
      extracellular ATP signal levels may be differently influenced by intracellular
      feedback pathways.
FAU - Vlajkovic, S M
AU  - Vlajkovic SM
AD  - Faculty of Medicine and Health Science, Department of Physiology, The University 
      of Auckland, Private Bag 92019, Auckland, New Zealand.
FAU - Housley, G D
AU  - Housley GD
FAU - Greenwood, D
AU  - Greenwood D
FAU - Thorne, P R
AU  - Thorne PR
LA  - eng
SI  - GENBANK/AF129103
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Purinergic)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (ectoATPase)
RN  - EC 3.6.1.5 (Apyrase)
SB  - IM
MH  - Adenosine Triphosphatases/*genetics/physiology
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apyrase/genetics
MH  - Base Sequence
MH  - Cochlea/enzymology
MH  - Gene Expression Regulation, Enzymologic
MH  - Isoenzymes/genetics/physiology
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Purinergic/*physiology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - *Synaptic Transmission
MH  - Tissue Distribution
EDAT- 1999/12/03 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/03 09:00
PHST- 1999/12/03 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/03 09:00 [entrez]
AID - S0169328X99002442 [pii]
AID - 10.1016/s0169-328x(99)00244-2 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Nov 10;73(1-2):85-92. doi:
      10.1016/s0169-328x(99)00244-2.