PMID- 10581288
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 153
IP  - 4
DP  - 1999 Dec
TI  - A selective screen reveals discrete functional domains in Drosophila Nanos.
PG  - 1825-38
AB  - The Drosophila protein Nanos encodes an evolutionarily conserved protein with two 
      zinc finger motifs. In the embryo, Nanos protein function is required for 
      establishment of the anterior-posterior body pattern and for the migration of 
      primordial germ cells. During oogenesis, Nanos protein is involved in the 
      establishment and maintenance of germ-line stem cells and the differentiation of 
      oocyte precursor cells. To establish proper embryonic patterning, Nanos acts as a 
      translational regulator of hunchback RNA. Nanos' targets for germ cell migration 
      and development are not known. Here, we describe a selective genetic screen aimed 
      at isolating new nanos alleles. The molecular and genetic analysis of 68 new 
      alleles has allowed us to identify amino acids critical for nanos function. This 
      analysis shows that the CCHC motifs, which coordinate two metal ions, are 
      essential for all known functions of Nanos protein. Furthermore, a region 
      C-terminal to the zinc fingers seems to constitute a novel functional domain 
      within the Nanos protein. This "tail region" of Nanos is required for abdomen 
      formation and germ cell migration, but not for oogenesis.
FAU - Arrizabalaga, G
AU  - Arrizabalaga G
AD  - Biology Department, Massachusetts Institute of Technology, Cambridge, 
      Massachusetts 02139-4307, USA.
FAU - Lehmann, R
AU  - Lehmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 142661-95-8 (nos protein, Drosophila)
SB  - IM
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Movement/genetics
MH  - Drosophila/*genetics/metabolism
MH  - *Drosophila Proteins
MH  - Female
MH  - Insect Proteins/*genetics/physiology
MH  - Male
MH  - Molecular Sequence Data
MH  - Morphogenesis/physiology
MH  - Phenotype
MH  - *RNA-Binding Proteins
MH  - Sequence Homology, Amino Acid
MH  - Zinc Fingers
PMC - PMC1460875
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - 10.1093/genetics/153.4.1825 [doi]
PST - ppublish
SO  - Genetics. 1999 Dec;153(4):1825-38. doi: 10.1093/genetics/153.4.1825.