PMID- 10581177
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 266
IP  - 1
DP  - 1999 Dec 9
TI  - Cyclic AMP inhibits the activity of c-Jun N-terminal kinase (JNKp46) but not
      JNKp55 and ERK2 in human helper T lymphocytes.
PG  - 129-34
AB  - The cyclic AMP (cAMP) elevating agent PGE(2) and dibutyryl cyclic AMP (dBcAMP)
      affect T cell functions. Using human helper T cell clones, we examined effects of
      cAMP on c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase
      (ERK), which are assumed to play a role in T cell regulation. When we analyzed
      the effects of dBcAMP on activities of mitogen-activated protein kinase (MAPK)
      family members ERK2, JNKp55 and JNKp46, dBcAMP did not inhibit the activities of 
      ERK2 and JNKp55 induced by PMA/A23187 stimulation. JNKp46 activity was, however, 
      inhibited by dBcAMP. JNK phosphorylates c-Jun on Ser-63 and Ser-73, the result
      being induction of its transcriptional activity. We found that dBcAMP inhibited
      the phosphorylation of c-Jun Ser-63 induced by PMA/A23187 stimulation. We suggest
      a different mechanism of regulation of JNKp55 and JNKp46 activities and that
      JNKp46 is a specific c-Jun kinase by which the activity of c-Jun is regulated in 
      T lymphocytes.
CI  - Copyright 1999 Academic Press.
FAU - Harada, Y
AU  - Harada Y
AD  - Department of Molecular and Developmental Biology, Institute of Medical Science, 
      University of Tokyo, Tokyo, Japan.
FAU - Miyatake, S
AU  - Miyatake S
FAU - Arai, K
AU  - Arai K
FAU - Watanabe, S
AU  - Watanabe S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 17885-08-4 (Phosphoserine)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Antibodies/immunology
MH  - Bucladesine/pharmacology
MH  - Calcimycin/pharmacology
MH  - Cell Line
MH  - Cyclic AMP/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mitogen-Activated Protein Kinase 1/*metabolism
MH  - Mitogen-Activated Protein Kinases/*antagonists &
      inhibitors/chemistry/immunology/*metabolism
MH  - Molecular Weight
MH  - Myelin Basic Protein/metabolism
MH  - Phosphorylation/drug effects
MH  - Phosphoserine/metabolism
MH  - Precipitin Tests
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Signal Transduction/drug effects
MH  - T-Lymphocytes, Helper-Inducer/drug effects/*enzymology/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - 10.1006/bbrc.1999.1783 [doi]
AID - S0006291X99917837 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Dec 9;266(1):129-34. doi:
      10.1006/bbrc.1999.1783.