PMID- 10580106
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 462
IP  - 1-2
DP  - 1999 Nov 26
TI  - Human napsin A: expression, immunochemical detection, and tissue localization.
PG  - 135-9
AB  - A novel aspartic proteinase, called napsin, has recently been found in human and 
      mouse. Due to high similarity with cathepsin D a structural model of human napsin
      A could be built. Based on this model a potential epitope SFYLNRDPEEPDGGE has
      been identified, which was used to immunize rabbits. The resulting antibody was
      employed in monitoring the expression of recombinant human napsin A in HEK293
      cell line. Western blot analysis confirmed the specificity of the antibody and
      showed that human napsin A is expressed as a single chain protein with the
      molecular weight of approximately 38 kDa. Immunohistochemical studies revealed
      high expression levels of napsin A in human kidney and lung but low expression in
      spleen.
FAU - Schauer-Vukasinovic, V
AU  - Schauer-Vukasinovic V
AD  - F. Hoffmann-La Roche Ltd., Pharma Division, Preclinical Research,
      Grenzacherstrasse 124, CH-4070, Basel, Switzerland.
FAU - Bur, D
AU  - Bur D
FAU - Kling, D
AU  - Kling D
FAU - Gruninger, F
AU  - Gruninger F
FAU - Giller, T
AU  - Giller T
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.23.- (NAPSA protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies/immunology
MH  - Aspartic Acid Endopeptidases/*analysis/immunology/metabolism
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Epitopes
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney/enzymology
MH  - Lung/enzymology
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/12/02 00:00
MHDA- 1999/12/02 00:01
CRDT- 1999/12/02 00:00
PHST- 1999/12/02 00:00 [pubmed]
PHST- 1999/12/02 00:01 [medline]
PHST- 1999/12/02 00:00 [entrez]
AID - S0014-5793(99)01458-1 [pii]
AID - 10.1016/s0014-5793(99)01458-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Nov 26;462(1-2):135-9. doi: 10.1016/s0014-5793(99)01458-1.