PMID- 10580105
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 462
IP  - 1-2
DP  - 1999 Nov 26
TI  - Napsin A, a member of the aspartic protease family, is abundantly expressed in
      normal lung and kidney tissue and is expressed in lung adenocarcinomas.
PG  - 129-34
AB  - A pair of 35 kDa polypeptides (TAO1/TAO2) are expressed in more than 90% of all
      primary lung adenocarcinomas but not in other major malignancies. Mass
      spectrometry of tryptic peptides showed that TAO1/TAO2 is identical to napsin A, 
      a recently described member of the aspartic proteinase family. The site of
      processing of pronapsin A to the mature form was located. Napsin expression was
      detected in human lung adenocarcinoma tumors, compatible with the marker nature
      of TAO1/TAO2 in the diagnosis of primary lung adenocarcinoma. This is important
      since identification of markers which can distinguish primary lung
      adenocarcinomas from distant metastases is desirable. Northern blot analysis
      showed expression of napsin also in normal lung and kidney tissue, and in situ
      hybridization showed expression in type II alveolar cells of the lung. This
      protease is concluded to have a specific functional role in the normal alveolar
      epithelium and is a candidate protease for the proteolytic processing of
      surfactant precursors.
FAU - Chuman, Y
AU  - Chuman Y
AD  - Laboratory of Cell Biology, National Cancer Institute, National Institutes of
      Health, Bethesda, MD 20892, USA.
FAU - Bergman, A
AU  - Bergman A
FAU - Ueno, T
AU  - Ueno T
FAU - Saito, S
AU  - Saito S
FAU - Sakaguchi, K
AU  - Sakaguchi K
FAU - Alaiya, A A
AU  - Alaiya AA
FAU - Franzen, B
AU  - Franzen B
FAU - Bergman, T
AU  - Bergman T
FAU - Arnott, D
AU  - Arnott D
FAU - Auer, G
AU  - Auer G
FAU - Appella, E
AU  - Appella E
FAU - Jornvall, H
AU  - Jornvall H
FAU - Linder, S
AU  - Linder S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Apoproteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Pulmonary Surfactant-Associated Proteins)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (RNA, Messenger)
RN  - 0 (pulmonary surfactant apoprotein)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.23.- (NAPSA protein, human)
SB  - IM
MH  - Adenocarcinoma/*enzymology
MH  - Amino Acid Sequence
MH  - Apoproteins/metabolism
MH  - Aspartic Acid Endopeptidases/*biosynthesis/genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis
MH  - Humans
MH  - Kidney/*enzymology
MH  - Lung/*enzymology
MH  - Lung Neoplasms/*enzymology
MH  - Molecular Sequence Data
MH  - *Pulmonary Surfactant-Associated Proteins
MH  - Pulmonary Surfactants/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Tissue Distribution
EDAT- 1999/12/02 00:00
MHDA- 1999/12/02 00:01
CRDT- 1999/12/02 00:00
PHST- 1999/12/02 00:00 [pubmed]
PHST- 1999/12/02 00:01 [medline]
PHST- 1999/12/02 00:00 [entrez]
AID - S0014579399014933 [pii]
AID - 10.1016/s0014-5793(99)01493-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Nov 26;462(1-2):129-34. doi: 10.1016/s0014-5793(99)01493-3.