PMID- 10580059
OWN - NLM
STAT- MEDLINE
DCOM- 19991215
LR  - 20200217
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 80 ( Pt 11)
DP  - 1999 Nov
TI  - An analysis of the role of neuraminidase in the receptor-binding activity of
      influenza B virus: the inhibitory effect of Zanamivir on haemadsorption.
PG  - 2969-2976
LID - 10.1099/0022-1317-80-11-2969 [doi]
AB  - We analysed the role of neuraminidase (NA) on haemadsorption by the
      haemagglutinin (HA) protein of influenza B virus. The influenza B virus mutant
      ts-7 has a temperature-sensitive mutation in the NA protein. At high temperature,
      cells infected with this virus did not exhibit haemadsorption activity, but the
      addition of bacterial neuraminidase (bNA) restored haemadsorption activity. COS
      cells transfected with HA cDNAs of B/Kanagawa/73 or B/Lee/40 virus showed no
      evidence of haemadsorption. However, with the addition of bNA or co- transfection
      with NA cDNA of the B/Lee/40 virus, haemadsorption was observed. Experiments with
      point-mutated HA cDNAs of B/Lee/40 virus showed that two N-acetyl glycosylation
      sites at amino acid residues 160 and 217 were responsible for the inability of
      the HA protein to adsorb to erythrocytes. These results indicated that
      haemadsorption by the HA protein of influenza B virus required the involvement of
      NA. Because the NA inhibitor Zanamivir was reported not to penetrate cells, we
      investigated the action of this inhibitor and found that Zanamivir inhibited
      haemadsorption on MDCK cells infected with B/Kanagawa/73 or B/Lee/40 virus. After
      removing Zanamivir by washing, the addition of bNA restored the haemadsorption
      activity on the infected cells. Scanning electron microscopy indicated that at
      0.4 microM Zanamivir, HA protein did not adsorb to erythrocytes but retained the 
      ability to aggregate virions. However, at 4 microM Zanamivir, distinct virion
      formation could not be observed.
FAU - Luo, C
AU  - Luo C
AD  - Department of Virology, Medical School, Nagoya City University, 1 Kawasumi,
      Mizuho-chou, Mizuho-ku, Nagoya 467, Japan 1.
FAU - Nobusawa, E
AU  - Nobusawa E
AD  - Department of Virology, Medical School, Nagoya City University, 1 Kawasumi,
      Mizuho-chou, Mizuho-ku, Nagoya 467, Japan 1.
FAU - Nakajima, K
AU  - Nakajima K
AD  - Department of Virology, Medical School, Nagoya City University, 1 Kawasumi,
      Mizuho-chou, Mizuho-ku, Nagoya 467, Japan 1.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Guanidines)
RN  - 0 (Hemagglutinin Glycoproteins, Influenza Virus)
RN  - 0 (Pyrans)
RN  - 0 (Receptors, Virus)
RN  - 0 (Sialic Acids)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - L6O3XI777I (Zanamivir)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - COS Cells
MH  - Chickens
MH  - Enzyme Inhibitors/*pharmacology
MH  - Erythrocytes/*virology
MH  - Geese
MH  - Glycosylation
MH  - Guanidines
MH  - Hemagglutinin Glycoproteins, Influenza Virus/*physiology
MH  - Influenza B virus/*physiology
MH  - Neuraminidase/antagonists & inhibitors/*physiology
MH  - Pyrans
MH  - Receptors, Virus/*physiology
MH  - Sialic Acids/*pharmacology
MH  - Virion/physiology
MH  - Zanamivir
EDAT- 1999/12/02 00:00
MHDA- 1999/12/02 00:01
CRDT- 1999/12/02 00:00
PHST- 1999/12/02 00:00 [pubmed]
PHST- 1999/12/02 00:01 [medline]
PHST- 1999/12/02 00:00 [entrez]
AID - 10.1099/0022-1317-80-11-2969 [doi]
PST - ppublish
SO  - J Gen Virol. 1999 Nov;80 ( Pt 11):2969-2976. doi: 10.1099/0022-1317-80-11-2969.