PMID- 10580002
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 22
DP  - 1999 Nov 15
TI  - Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases.
PG  - 2946-57
AB  - Activating phosphorylation of cyclin-dependent protein kinases (CDKs) is
      necessary for their kinase activity and cell cycle progression. This
      phosphorylation is carried out by the Cdk-activating kinase (CAK); in contrast,
      little is known about the corresponding protein phosphatase. We show that type 2C
      protein phosphatases (PP2Cs) are responsible for this dephosphorylation of
      Cdc28p, the major budding yeast CDK. Two yeast PP2Cs, Ptc2p and Ptc3p, display
      Cdc28p phosphatase activity in vitro and in vivo, and account for approximately
      90% of Cdc28p phosphatase activity in yeast extracts. Overexpression of PTC2 or
      PTC3 results in synthetic lethality in strains temperature-sensitive for yeast
      CAK1, and disruptions of PTC2 and PTC3 suppress the growth defect of a cak1
      mutant. Furthermore, PP2C-like enzymes are the predominant phosphatases toward
      human Cdk2 in HeLa cell extracts, indicating that the substrate specificity of
      PP2Cs toward CDKs is evolutionarily conserved.
FAU - Cheng, A
AU  - Cheng A
AD  - Department of Molecular Biophysics and Biochemistry, Yale University School of
      Medicine, New Haven, Connecticut 06520-8024, USA.
FAU - Ross, K E
AU  - Ross KE
FAU - Kaldis, P
AU  - Kaldis P
FAU - Solomon, M J
AU  - Solomon MJ
LA  - eng
GR  - R01 GM047830/GM/NIGMS NIH HHS/United States
GR  - GM47830/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Fungal Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 1114-81-4 (Phosphothreonine)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDC28 Protein Kinase, S cerevisiae)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - EC 2.7.11.22 (cyclin-dependent kinase-activating kinase)
RN  - EC 3.1.3.16 (PTC1 protein, S cerevisiae)
RN  - EC 3.1.3.16 (PTC3 protein, S cerevisiae)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
RN  - EC 3.1.3.16 (Protein Phosphatase 2C)
SB  - IM
MH  - CDC28 Protein Kinase, S cerevisiae/metabolism
MH  - Cell Cycle
MH  - Cyclin-Dependent Kinases/*metabolism
MH  - Enzyme Activation
MH  - Fungal Proteins/*metabolism
MH  - Gene Expression Regulation, Fungal
MH  - Humans
MH  - Phosphoprotein Phosphatases/*metabolism
MH  - Phosphorylation
MH  - Phosphothreonine/metabolism
MH  - Protein Phosphatase 2
MH  - Protein Phosphatase 2C
MH  - *Protein Processing, Post-Translational
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Species Specificity
PMC - PMC317162
EDAT- 1999/12/02 00:00
MHDA- 1999/12/02 00:01
CRDT- 1999/12/02 00:00
PHST- 1999/12/02 00:00 [pubmed]
PHST- 1999/12/02 00:01 [medline]
PHST- 1999/12/02 00:00 [entrez]
AID - 10.1101/gad.13.22.2946 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Nov 15;13(22):2946-57. doi: 10.1101/gad.13.22.2946.