PMID- 10579726
OWN - NLM
STAT- MEDLINE
DCOM- 19991230
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 147
IP  - 5
DP  - 1999 Nov 29
TI  - Enhancement of endothelial cell migration and in vitro tube formation by TAP20, a
      novel beta 5 integrin-modulating, PKC theta-dependent protein.
PG  - 1073-84
AB  - Migration, proliferation, and tube formation of endothelial cells are regulated
      by a protein kinase C isoenzyme PKCtheta. A full-length cDNA encoding a novel
      20-kD protein, whose expression was PKCtheta-dependent, was identified in
      endothelial cells, cloned, characterized, and designated as theta-associated
      protein (TAP) 20. Overexpression of TAP20 decreased cell adhesion and enhanced
      migration on vitronectin and tube formation in three-dimensional culture. An
      antiintegrin alphavbeta5 antibody prevented these TAP20 effects. Overexpression
      of TAP20 also decreased focal adhesion formation in alphavbeta3-deficient cells. 
      The interaction between TAP20 and beta5 integrin cytoplasmic domain was
      demonstrated by protein coprecipitation and immunoblotting. Thus, the discovery
      of TAP20, which interacts with integrin beta5 and modulates cell adhesion,
      migration, and tube formation, further defines a possible pathway to angiogenesis
      dependent on PKCtheta.
FAU - Tang, S
AU  - Tang S
AD  - Cardiovascular Division, Department of Medicine, Department of Molecular
      Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
      tang@aecom.yu.edu
FAU - Gao, Y
AU  - Gao Y
FAU - Ware, J A
AU  - Ware JA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (ITGB3BP protein, human)
RN  - 0 (Integrin beta Chains)
RN  - 0 (Integrins)
RN  - 0 (Isoenzymes)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Vitronectin)
RN  - 0 (TAP20 protein, rat)
RN  - 0 (integrin beta5)
RN  - EC 2.7.11.13 (PRKCQ protein, human)
RN  - EC 2.7.11.13 (Pkcq protein, rat)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C-theta)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Adhesion/physiology
MH  - Cell Division/physiology
MH  - Cell Movement/*physiology
MH  - Cloning, Molecular
MH  - Cytoplasm/chemistry
MH  - Endothelium, Vascular/cytology/physiology
MH  - Humans
MH  - *Integrin beta Chains
MH  - Integrins/metabolism/*physiology
MH  - Isoenzymes/*physiology
MH  - Membrane Glycoproteins/genetics/*physiology
MH  - Molecular Sequence Data
MH  - Neovascularization, Physiologic/physiology
MH  - Nuclear Proteins
MH  - Protein Kinase C/*physiology
MH  - Protein Kinase C-theta
MH  - Proteins/chemistry
MH  - Rats
MH  - Receptors, Vitronectin/metabolism
MH  - Tumor Cells, Cultured
MH  - Zinc Fingers
PMC - PMC2169340
EDAT- 1999/12/01 00:00
MHDA- 1999/12/01 00:01
CRDT- 1999/12/01 00:00
PHST- 1999/12/01 00:00 [pubmed]
PHST- 1999/12/01 00:01 [medline]
PHST- 1999/12/01 00:00 [entrez]
AID - 10.1083/jcb.147.5.1073 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Nov 29;147(5):1073-84. doi: 10.1083/jcb.147.5.1073.