PMID- 10576742
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20191210
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 286
IP  - 5445
DP  - 1999 Nov 26
TI  - Phosphorylation and regulation of Raf by Akt (protein kinase B).
PG  - 1741-4
AB  - Activation of the protein kinase Raf can lead to opposing cellular responses such
      as proliferation, growth arrest, apoptosis, or differentiation. Akt (protein
      kinase B), a member of a different signaling pathway that also regulates these
      responses, interacted with Raf and phosphorylated this protein at a highly
      conserved serine residue in its regulatory domain in vivo. This phosphorylation
      of Raf by Akt inhibited activation of the Raf-MEK-ERK signaling pathway and
      shifted the cellular response in a human breast cancer cell line from cell cycle 
      arrest to proliferation. These observations provide a molecular basis for cross
      talk between two signaling pathways at the level of Raf and Akt.
FAU - Zimmermann, S
AU  - Zimmermann S
AD  - Institute of Medical Virology, University of Zurich, Gloriastrasse 30/32, CH-8028
      Zurich, Switzerland.
FAU - Moelling, K
AU  - Moelling K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Chromones)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Morpholines)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Somatomedins)
RN  - 17885-08-4 (Phosphoserine)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.11.1 (AKT1 protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-raf)
RN  - EC 3.6.5.2 (ras Proteins)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - *Cell Division
MH  - Cell Line
MH  - Chromones/pharmacology
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/metabolism
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Epidermal Growth Factor/pharmacology
MH  - Flavonoids/pharmacology
MH  - Humans
MH  - *MAP Kinase Signaling System
MH  - Morpholines/pharmacology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Protein-Serine-Threonine Kinases/antagonists & inhibitors/*metabolism
MH  - Proto-Oncogene Proteins/antagonists & inhibitors/*metabolism
MH  - Proto-Oncogene Proteins c-akt
MH  - Proto-Oncogene Proteins c-raf/antagonists & inhibitors/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Signal Transduction
MH  - Somatomedins/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Cells, Cultured
MH  - ras Proteins/metabolism
EDAT- 1999/11/27 00:00
MHDA- 1999/11/27 00:01
CRDT- 1999/11/27 00:00
PHST- 1999/11/27 00:00 [pubmed]
PHST- 1999/11/27 00:01 [medline]
PHST- 1999/11/27 00:00 [entrez]
AID - 8027 [pii]
AID - 10.1126/science.286.5445.1741 [doi]
PST - ppublish
SO  - Science. 1999 Nov 26;286(5445):1741-4. doi: 10.1126/science.286.5445.1741.