PMID- 10574968
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 49
DP  - 1999 Dec 3
TI  - Balance between activities of Rho kinase and type 1 protein phosphatase modulates
      turnover of phosphorylation and dynamics of desmin/vimentin filaments.
PG  - 34932-9
AB  - To analyze the cell cycle-dependent desmin phosphorylation by Rho kinase, we
      developed antibodies specifically recognizing the kinase-dependent
      phosphorylation of desmin at Thr-16, Thr-75, and Thr-76. With these antibodies,
      phosphorylation of desmin was observed specifically at the cleavage furrow in
      late mitotic Saos-2 cells. We then found that treatment of the interphase cells
      with calyculin A revealed phosphorylation at all the three sites of desmin. We
      also found that an antibody, which specifically recognizes vimentin
      phosphorylated at Ser-71 by Rho kinase, became immunoreactive after calyculin A
      treatment. This calyculin A-induced interphase phosphorylation of vimentin at
      Ser-71 was blocked by Rho kinase inhibitor or by expression of the
      dominant-negative Rho kinase. Taken together, our results indicate that Rho
      kinase is activated not only in mitotic cells but also interphase ones, and
      phosphorylates intermediate filament proteins, although the apparent
      phosphorylation level is diminished to an undetectable level due to the
      constitutive action of type 1 protein phosphatase. The balance between
      intermediate filament protein phosphorylation by Rho kinase and dephosphorylation
      by type 1 protein phosphatase may affect the continuous exchange of intermediate 
      filament subunits between a soluble pool and polymerized intermediate filaments.
FAU - Inada, H
AU  - Inada H
AD  - Division of Biochemistry, Aichi Cancer Center Research Institute, 1-1 Chikusa-ku,
      Nagoya, Aichi 464-8681, Japan.
FAU - Togashi, H
AU  - Togashi H
FAU - Nakamura, Y
AU  - Nakamura Y
FAU - Kaibuchi, K
AU  - Kaibuchi K
FAU - Nagata, K
AU  - Nagata K
FAU - Inagaki, M
AU  - Inagaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Desmin)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Marine Toxins)
RN  - 0 (Oxazoles)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vimentin)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - 7D07U14TK3 (calyculin A)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle
MH  - Cell Line
MH  - Desmin/immunology/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Humans
MH  - Immunoblotting
MH  - Intracellular Signaling Peptides and Proteins
MH  - Marine Toxins
MH  - Mice
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Okadaic Acid/pharmacology
MH  - Oxazoles/pharmacology
MH  - Phosphoprotein Phosphatases/*metabolism
MH  - Phosphorylation/drug effects
MH  - Protein-Serine-Threonine Kinases/antagonists &
      inhibitors/immunology/*metabolism/physiology
MH  - Recombinant Proteins/metabolism
MH  - Time Factors
MH  - Vimentin/immunology/*metabolism
MH  - rho-Associated Kinases
EDAT- 1999/11/27 00:00
MHDA- 1999/11/27 00:01
CRDT- 1999/11/27 00:00
PHST- 1999/11/27 00:00 [pubmed]
PHST- 1999/11/27 00:01 [medline]
PHST- 1999/11/27 00:00 [entrez]
AID - 10.1074/jbc.274.49.34932 [doi]
AID - S0021-9258(19)53411-1 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 3;274(49):34932-9. doi: 10.1074/jbc.274.49.34932.