PMID- 10574931
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 49
DP  - 1999 Dec 3
TI  - Functional interaction between SHPTP1 and the Lyn tyrosine kinase in the
      apoptotic response to DNA damage.
PG  - 34663-8
AB  - The Lyn protein-tyrosine kinase is activated in the cellular response to
      DNA-damaging agents. Here we demonstrate that Lyn associates constitutively with 
      the SHPTP1 protein-tyrosine phosphatase. The SH3 domain of Lyn interacts directly
      with SHPTP1. The results show that Lyn phosphorylates SHPTP1 at the C-terminal
      Tyr-564 site. Lyn-mediated phosphorylation of SHPTP1 stimulates SHPTP1 tyrosine
      phosphatase activity. We also demonstrate that treatment of cells with
      1-beta-D-arabinofuranosylcytosine and other genotoxic agents induces
      Lyn-dependent phosphorylation and activation of SHPTP1. The significance of the
      Lyn-SHPTP1 interaction is supported by the demonstration that activation of Lyn
      contributes in part to the apoptotic response to ara-C treatment and that SHPTP1 
      attenuates this response. These findings support a functional interaction between
      Lyn and SHPTP1 in the response to DNA damage.
FAU - Yoshida, K
AU  - Yoshida K
AD  - Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts
      02115, USA.
FAU - Kharbanda, S
AU  - Kharbanda S
FAU - Kufe, D
AU  - Kufe D
LA  - eng
GR  - CA29431/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 04079A1RDZ (Cytarabine)
RN  - EC 2.7.10.2 (lyn protein-tyrosine kinase)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.1.3.48 (PTPN11 protein, human)
RN  - EC 3.1.3.48 (PTPN6 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 6)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - *Apoptosis
MH  - Cell Cycle
MH  - Cytarabine/pharmacology
MH  - *DNA Damage
MH  - Down-Regulation
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Phosphorylation/drug effects
MH  - Precipitin Tests
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 6
MH  - Protein Tyrosine Phosphatases/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Time Factors
MH  - Transfection
MH  - U937 Cells
MH  - src-Family Kinases/*metabolism
EDAT- 1999/11/27 00:00
MHDA- 1999/11/27 00:01
CRDT- 1999/11/27 00:00
PHST- 1999/11/27 00:00 [pubmed]
PHST- 1999/11/27 00:01 [medline]
PHST- 1999/11/27 00:00 [entrez]
AID - 10.1074/jbc.274.49.34663 [doi]
AID - S0021-9258(19)53374-9 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 3;274(49):34663-8. doi: 10.1074/jbc.274.49.34663.