PMID- 10574923
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 49
DP  - 1999 Dec 3
TI  - Autoantibodies define a family of proteins with conserved double-stranded
      RNA-binding domains as well as DNA binding activity.
PG  - 34598-604
AB  - Cellular responses to viral infection are signaled by double-stranded (ds) RNA,
      which is not found in substantial amounts in uninfected cells. Although cellular 
      dsRNA-binding proteins have been described, their characterization is incomplete.
      We show that dsRNA-binding proteins are prominent autoantigens. Sera from B6 and 
      B10.S mice with pristane-induced lupus and human autoimmune sera
      immunoprecipitated a novel set of 130-, 110-, 90-, 80-, and 45-kDa proteins. The 
      proteins were all major cellular poly(IC)-binding factors. N-terminal amino acid 
      sequences of p110 and p90 were identical and matched nuclear factor (NF) 90 and M
      phase phosphoprotein 4. p45 and p90 were identified as the NF45.NF90 complex,
      which binds the interleukin-2 promoter as well as certain highly structured viral
      RNAs. NF90.NF45 and M phase phosphoprotein 4 belong to a large group of proteins 
      with conserved dsRNA-binding motifs. Besides binding dsRNA, NF90.NF45, p110, and 
      p130 had single-stranded and dsDNA binding activity. Some sera contained
      autoantibodies whose binding was inhibited by poly(IC) but not single-stranded
      DNA or vice versa, suggesting that the DNA- and RNA-binding sites are different. 
      These autoantibodies will be useful probes of the function of dsRNA-binding
      proteins. Their interaction with dsRNA, an immunological adjuvant, also could
      promote autoimmunity.
FAU - Satoh, M
AU  - Satoh M
AD  - Department of Medicine, Lineberger Comprehensive Cancer Center, University of
      North Carolina, Chapel Hill, North Carolina 27599-7280, USA.
FAU - Shaheen, V M
AU  - Shaheen VM
FAU - Kao, P N
AU  - Kao PN
FAU - Okano, T
AU  - Okano T
FAU - Shaw, M
AU  - Shaw M
FAU - Yoshida, H
AU  - Yoshida H
FAU - Richards, H B
AU  - Richards HB
FAU - Reeves, W H
AU  - Reeves WH
LA  - eng
GR  - AR40391/AR/NIAMS NIH HHS/United States
GR  - P60-AR30701/AR/NIAMS NIH HHS/United States
GR  - R01-AR44731/AR/NIAMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Autoantibodies)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Viral)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Autoantibodies/*immunology/*metabolism
MH  - Binding Sites
MH  - Chromatography, Affinity
MH  - DNA/immunology/metabolism
MH  - DNA-Binding Proteins/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoblotting
MH  - K562 Cells
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Double-Stranded/immunology/metabolism
MH  - RNA, Viral/immunology
MH  - RNA-Binding Proteins/*immunology
MH  - Recombinant Proteins/immunology/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
EDAT- 1999/11/27 00:00
MHDA- 1999/11/27 00:01
CRDT- 1999/11/27 00:00
PHST- 1999/11/27 00:00 [pubmed]
PHST- 1999/11/27 00:01 [medline]
PHST- 1999/11/27 00:00 [entrez]
AID - 10.1074/jbc.274.49.34598 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 3;274(49):34598-604. doi: 10.1074/jbc.274.49.34598.