PMID- 10574912
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 49
DP  - 1999 Dec 3
TI  - Cyclin K functions as a CDK9 regulatory subunit and participates in RNA
      polymerase II transcription.
PG  - 34527-30
AB  - Important progress in the understanding of elongation control by RNA polymerase
      II (RNAPII) has come from the recent identification of the positive transcription
      elongation factor b (P-TEFb) and the demonstration that this factor is a protein 
      kinase that phosphorylates the carboxyl-terminal domain (CTD) of the RNAPII
      largest subunit. The P-TEFb complex isolated from mammalian cells contains a
      catalytic subunit (CDK9), a cyclin subunit (cyclin T1 or cyclin T2), and
      additional, yet unidentified, polypeptides of unknown function. To identify
      additional factors involved in P-TEFb function we performed a yeast two-hybrid
      screen using CDK9 as bait and found that cyclin K interacts with CDK9 in vivo.
      Biochemical analyses indicate that cyclin K functions as a regulatory subunit of 
      CDK9. The CDK9-cyclin K complex phosphorylated the CTD of RNAPII and functionally
      substituted for P-TEFb comprised of CDK9 and cyclin T in in vitro transcription
      reactions.
FAU - Fu, T J
AU  - Fu TJ
AD  - Department of Biology, Tularik Inc., South San Francisco, California 92080, USA.
FAU - Peng, J
AU  - Peng J
FAU - Lee, G
AU  - Lee G
FAU - Price, D H
AU  - Price DH
FAU - Flores, O
AU  - Flores O
LA  - eng
GR  - AI46391/AI/NIAID NIH HHS/United States
GR  - GM 35500/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCNK protein, human)
RN  - 0 (Cyclins)
RN  - EC 2.7.11.22 (CDK9 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 9)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Chromatography, Gel
MH  - Cyclin-Dependent Kinase 9
MH  - Cyclin-Dependent Kinases/metabolism/*physiology
MH  - Cyclins/metabolism/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation, Enzymologic
MH  - HeLa Cells
MH  - Humans
MH  - Kinetics
MH  - Protein Binding
MH  - RNA Polymerase II/*metabolism
MH  - *Transcription, Genetic
MH  - Two-Hybrid System Techniques
EDAT- 1999/11/27 00:00
MHDA- 1999/11/27 00:01
CRDT- 1999/11/27 00:00
PHST- 1999/11/27 00:00 [pubmed]
PHST- 1999/11/27 00:01 [medline]
PHST- 1999/11/27 00:00 [entrez]
AID - 10.1074/jbc.274.49.34527 [doi]
AID - S0021-9258(19)53355-5 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 3;274(49):34527-30. doi: 10.1074/jbc.274.49.34527.