PMID- 10574770
OWN - NLM
STAT- MEDLINE
DCOM- 20000511
LR  - 20190728
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 22
DP  - 1999 Nov 18
TI  - A role of the cryptic gene in the correct establishment of the left-right axis.
PG  - 1339-42
AB  - During vertebrate embryogenesis, a left-right axis is established. The heart,
      associated vessels and inner organs adopt asymmetric spatial arrangements and
      morphologies. Secreted growth factors of the TGF-beta family, including nodal,
      lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries
      [1] [2] [3]. In zebrafish, nodal signalling requires the presence of one-eyed
      pinhead (oep), a member of the EGF-CFC family of membrane-associated proteins
      [4]. We have generated a mutant allele of cryptic, a mouse EGF-CFC gene [5].
      Homozygous cryptic mutants developed to birth, but the majority died during the
      first week of life because of complex cardiac malformations such as
      malpositioning of the great arteries, and atrial-ventricular septal defects.
      Moreover, laterality defects, including right isomerism of the lungs, right or
      left positioning of the stomach and splenic hypoplasia were observed. Nodal gene 
      expression in the node was initiated in cryptic mutant mice, but neither nodal,
      lefty-2 nor Pitx2 were expressed in the left lateral plate mesoderm. The
      laterality defects observed in cryptic(-/-) mice resemble those of mice lacking
      the type IIB activin receptor or the homeobox-containing factor Pitx2 [6] [7] [8]
      [9], and are reminiscent of the human asplenic syndrome [10]. Our results provide
      genetic evidence for a role of cryptic in the signalling cascade that determines 
      left-right asymmetry.
FAU - Gaio, U
AU  - Gaio U
AD  - Max-Delbruck-Centrum for Molecular Medicine, Berlin, 13125, Germany.
FAU - Schweickert, A
AU  - Schweickert A
FAU - Fischer, A
AU  - Fischer A
FAU - Garratt, A N
AU  - Garratt AN
FAU - Muller, T
AU  - Muller T
FAU - Ozcelik, C
AU  - Ozcelik C
FAU - Lankes, W
AU  - Lankes W
FAU - Strehle, M
AU  - Strehle M
FAU - Britsch, S
AU  - Britsch S
FAU - Blum, M
AU  - Blum M
FAU - Birchmeier, C
AU  - Birchmeier C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (CFC1 protein, human)
RN  - 0 (Cfc1 protein, mouse)
RN  - 0 (Growth Substances)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (LEFTY1 protein, human)
RN  - 0 (Left-Right Determination Factors)
RN  - 0 (NODAL protein, human)
RN  - 0 (Nodal Protein)
RN  - 0 (Nodal protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (homeobox protein PITX1)
RN  - 0 (homeobox protein PITX3)
RN  - 0 (tdgf1 protein, zebrafish)
RN  - 184787-43-7 (homeobox protein PITX2)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Animals, Newborn
MH  - Dextrocardia/embryology/genetics
MH  - Embryonic and Fetal Development/*genetics
MH  - Fetal Heart/abnormalities
MH  - Gene Expression Regulation, Developmental
MH  - Genotype
MH  - Growth Substances/*physiology
MH  - Heart Defects, Congenital/embryology/genetics
MH  - Homeodomain Proteins/genetics/physiology
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Left-Right Determination Factors
MH  - Mesoderm/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Morphogenesis/*genetics
MH  - Nodal Protein
MH  - *Nuclear Proteins
MH  - Paired Box Transcription Factors
MH  - Recombinant Fusion Proteins/physiology
MH  - Signal Transduction/physiology
MH  - Spleen/abnormalities
MH  - Syndrome
MH  - Transcription Factors/genetics/physiology
MH  - Transforming Growth Factor beta/deficiency/genetics/physiology
MH  - Transposition of Great Vessels/embryology/genetics
MH  - Viscera/abnormalities
MH  - *Zebrafish Proteins
EDAT- 1999/11/27 09:00
MHDA- 2000/05/16 09:00
CRDT- 1999/11/27 09:00
PHST- 1999/11/27 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 1999/11/27 09:00 [entrez]
AID - S0960-9822(00)80059-7 [pii]
AID - 10.1016/s0960-9822(00)80059-7 [doi]
PST - ppublish
SO  - Curr Biol. 1999 Nov 18;9(22):1339-42. doi: 10.1016/s0960-9822(00)80059-7.