PMID- 10574709
OWN - NLM
STAT- MEDLINE
DCOM- 20000424
LR  - 20220409
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 24)
DP  - 1999 Dec
TI  - Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein 
      that induces plasma membrane extensions and increased motility in keratinocytes.
PG  - 4601-13
AB  - PA2.26 antigen was identified as a cell-surface protein induced in epidermal
      carcinogenesis and skin remodeling processes. PA2.26 is expressed in carcinoma
      cell lines and cultured fibroblasts but absent in nontumorigenic keratinocytes.
      In tissues, PA2.26 is present in epithelial cells of the choroid plexus,
      ependyma, glomerulus and alveolus, in mesothelial cells, and in endothelia of
      lymphatic vessels. Biochemical characterization of PA2.26 protein and sequence
      analysis of the isolated cDNA demonstrate that PA2.26 antigen is a mucin-like
      transmembrane glycoprotein. Confocal and immunoelectron microscopy analysis in
      cultured cells reveal that PA2. 26 is concentrated in actin-rich microvilli and
      plasma membrane projections, such as filopodia, lamellipodia and ruffles, where
      it colocalizes with members of the ERM (ezrin, radixin, moesin) family protein.
      Ezrin and moesin, but not radixin, can be coimmunoprecipitated together with
      PA2.26 from cell lysates. Ectopic expression of PA2.26 in immortalized,
      nontumorigenic, keratinocytes induces an epithelial-fibroblastoid morphological
      conversion with increased plasma membrane extensions, concomitantly to a major
      reorganization of the actin cytoskeleton, redistribution of ezrin to cell-surface
      projections, and enhanced motility. These findings suggest an involvement of
      PA2.26 in cell migration.
FAU - Scholl, F G
AU  - Scholl FG
AD  - Instituto de Investigaciones Biomedicas Alberto Sols CSIC-UAM, Hospital de la
      Princesa, Facultad de Medicina UAM, Spain.
FAU - Gamallo, C
AU  - Gamallo C
FAU - Vilaro, S
AU  - Vilaro S
FAU - Quintanilla, M
AU  - Quintanilla M
LA  - eng
SI  - GENBANK/AJ250246
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Actins)
RN  - 0 (Antigens, Surface)
RN  - 0 (DNA, Complementary)
RN  - 0 (Gp38 protein, mouse)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Actins/ultrastructure
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Surface/genetics/immunology/*metabolism
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Membrane/ultrastructure
MH  - Cell Movement/*immunology
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Immunohistochemistry
MH  - Keratinocytes/*cytology
MH  - Membrane Glycoproteins/genetics/immunology/*metabolism
MH  - Mice
MH  - Microscopy, Immunoelectron
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
EDAT- 1999/11/27 09:00
MHDA- 2000/04/29 09:00
CRDT- 1999/11/27 09:00
PHST- 1999/11/27 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 1999/11/27 09:00 [entrez]
AID - 10.1242/jcs.112.24.4601 [doi]
PST - ppublish
SO  - J Cell Sci. 1999 Dec;112 ( Pt 24):4601-13. doi: 10.1242/jcs.112.24.4601.