PMID- 10574510
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20191103
IS  - 1071-7323 (Print)
IS  - 1071-7323 (Linking)
VI  - 7
IP  - 6
DP  - 1999 Nov
TI  - Sequence variants in the human cocaine and amphetamine-regulated transcript
      (CART) gene in subjects with early onset obesity.
PG  - 532-6
AB  - OBJECTIVE: The cocaine and amphetamine-regulated transcript (CART) is expressed
      in the brain of rodents and humans, and intracerebroventricular injection of the 
      peptide in rats reduces food intake. The objective of the present study was to
      chromosomally map the CART gene and to examine the coding region of the gene for 
      variability in obese subjects. METHODS: The coding region of the CART gene was
      analyzed by single-strand conformation polymorphism analysis in 84 subjects with 
      early onset obesity. The prevalence of identified mutations was estimated in a
      cohort of 757 subjects with juvenile onset obesity [body mass index (BMI) =
      35.7+/-5.7 kg/m2+/-standard deviation (S)] and in 890 random control subjects
      (BMI = 26.1+/-3.6 kg/m2+/-S). Furthermore, using radiation hybrid mapping we
      mapped the chromosomal localization of the human CART gene. RESULTS: Radiation
      hybrid mapping co-localized the CART gene with a recently published human obesity
      locus at chromosome 5q13-14 corresponding also to an obesity locus at the similar
      syntenic region in mice. We identified two silent polymorphisms in the 3'UTR
      region of the gene (position 1457 deletion of A and position 1475 A-->G
      substitution) and the prevalence of these was determined among obese and control 
      subjects. However, none of the variants were associated with either obesity or
      weight gain during an average follow-up period of 27.4+/-8.4 years (S).
      CONCLUSION: Mutations in the coding region of the CART gene are unlikely to be
      involved in body weight control in Danish Caucasians with early onset obesity.
FAU - Echwald, S M
AU  - Echwald SM
AD  - Steno Diabetes Center and Hagedorn Research Institute, Copenhagen, Denmark.
      sme@hagedorn.dk
FAU - Sorensen, T I
AU  - Sorensen TI
FAU - Andersen, T
AU  - Andersen T
FAU - Hansen, C
AU  - Hansen C
FAU - Tommerup, N
AU  - Tommerup N
FAU - Pedersen, O
AU  - Pedersen O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obes Res
JT  - Obesity research
JID - 9305691
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Body Mass Index
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 5
MH  - Cohort Studies
MH  - Denmark
MH  - Genotype
MH  - Humans
MH  - Male
MH  - *Mutation
MH  - Nerve Tissue Proteins/*genetics
MH  - Obesity/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - Weight Gain
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1002/j.1550-8528.1999.tb00710.x [doi]
PST - ppublish
SO  - Obes Res. 1999 Nov;7(6):532-6. doi: 10.1002/j.1550-8528.1999.tb00710.x.