PMID- 10574461
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190513
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 6
IP  - 5
DP  - 1999 Oct 29
TI  - Characterization of cDNA clones selected by the GeneMark analysis from
      size-fractionated cDNA libraries from human brain.
PG  - 329-36
AB  - We have conducted a sequencing project of human cDNAs which encode large proteins
      in brain. For selection of cDNA clones to be sequenced in this project, cDNA
      clones have been experimentally examined by in vitro transcription/translation
      prior to sequencing. In this study, we tested an alternative approach for picking
      up cDNA clones having a high probability of carrying protein coding region. This 
      approach exploited 5'-end single-pass sequence data and the GeneMark program for 
      assessing protein-coding potential, and allowed us to select 74 clones out of
      14,804 redundant cDNA clones. The complete sequence data of these 74 clones
      revealed that 45% of them encoded proteins consisting of more than 500 amino acid
      residues while all the clones thus selected carried possible protein coding
      sequences as expected. The results indicated that the GeneMark analysis of 5'-end
      sequences of cDNAs offered us a simple and effective means to select cDNA clones 
      with protein-coding potential although the sizes of the encoded proteins could
      not be predicted.
FAU - Hirosawa, M
AU  - Hirosawa M
AD  - Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
FAU - Nagase, T
AU  - Nagase T
FAU - Ishikawa, K
AU  - Ishikawa K
FAU - Kikuno, R
AU  - Kikuno R
FAU - Nomura, N
AU  - Nomura N
FAU - Ohara, O
AU  - Ohara O
LA  - eng
SI  - GENBANK/AB032945
SI  - GENBANK/AB032946
SI  - GENBANK/AB032947
SI  - GENBANK/AB032948
SI  - GENBANK/AB032949
SI  - GENBANK/AB032950
SI  - GENBANK/AB032951
SI  - GENBANK/AB032952
SI  - GENBANK/AB032953
SI  - GENBANK/AB032954
SI  - GENBANK/AB032955
SI  - GENBANK/AB032956
SI  - GENBANK/AB032957
SI  - GENBANK/AB032958
SI  - GENBANK/AB032959
SI  - GENBANK/AB032960
SI  - GENBANK/AB032961
SI  - GENBANK/AB032962
SI  - GENBANK/AB032963
SI  - GENBANK/AB032964
SI  - GENBANK/AB032965
SI  - GENBANK/AB032966
SI  - GENBANK/AB032967
SI  - GENBANK/AB032968
SI  - GENBANK/AB032969
SI  - GENBANK/AB032970
SI  - GENBANK/AB032971
SI  - GENBANK/AB032972
SI  - GENBANK/AB032973
SI  - GENBANK/AB032974
SI  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes
      and genomes
JID - 9423827
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (Proteins)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Brain/*metabolism
MH  - *Cloning, Molecular
MH  - DNA, Complementary/*genetics
MH  - Gene Expression Profiling
MH  - Gene Library
MH  - Humans
MH  - Molecular Sequence Data
MH  - Physical Chromosome Mapping
MH  - Proteins/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA/*methods
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1093/dnares/6.5.329 [doi]
PST - ppublish
SO  - DNA Res. 1999 Oct 29;6(5):329-36. doi: 10.1093/dnares/6.5.329.