PMID- 10574203
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 734
IP  - 1
DP  - 1999 Oct 29
TI  - Carbohydrate release from picomole quantities of glycoprotein and
      characterisation of glycans by high-performance liquid chromatography and mass
      spectrometry.
PG  - 169-74
AB  - Samples of 5 to 20 microg of human IgG were subjected to dithiothreitol treatment
      to reduce disulphide bridges, followed by tryptic digestion. Glycans released
      from the tryptic peptide mixture by PNGase F digestion were then derivatised with
      2-aminoacridone. Labelled oligosaccharides were separated by normal-phase
      high-performance liquid chromatography and individual components were collected
      for matrix-assisted laser desorption ionization time-of-flight and electrospray
      mass spectrometric analysis.
FAU - Charlwood, J
AU  - Charlwood J
AD  - SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
FAU - Birrell, H
AU  - Birrell H
FAU - Camilleri, P
AU  - Camilleri P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Aminoacridines)
RN  - 0 (Disulfides)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Oligosaccharides)
RN  - 0 (Polysaccharides)
RN  - 27918-14-5 (2-aminoacridone)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.1.52 (Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Amidohydrolases/metabolism
MH  - Aminoacridines
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Disulfides/metabolism
MH  - Dithiothreitol/pharmacology
MH  - Glycoproteins/*metabolism
MH  - Humans
MH  - Immunoglobulin G/chemistry/*metabolism
MH  - Microchemistry
MH  - Oligosaccharides/analysis/isolation & purification
MH  - Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
MH  - Polysaccharides/chemistry/*isolation & purification/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Trypsin/metabolism
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1016/s0378-4347(99)00330-8 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Oct 29;734(1):169-74. doi:
      10.1016/s0378-4347(99)00330-8.