PMID- 10573531
OWN - NLM
STAT- MEDLINE
DCOM- 19991216
LR  - 20211203
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 30
IP  - 6
DP  - 1999 Dec
TI  - The human multidrug resistance protein 2 gene: functional characterization of the
      5'-flanking region and expression in hepatic cells.
PG  - 1507-12
AB  - The human multidrug resistance protein 2 (MRP2), also termed as the canalicular
      multispecific organic anion transporter (cMOAT), is a member of the adenosine
      triphosphate-binding cassette transporter superfamily. In the liver, MRP2
      mediates the multispecific efflux of various types of organic anions, including
      glucuronate, sulfate, and glutathione conjugates, across the canalicular
      hepatocyte membrane to the bile. To investigate how the MRP2 gene is expressed in
      liver cells, the 5'-flanking region of the human MRP2 gene was isolated from a
      human placental genomic library. Sequence analysis of the MRP2 promoter showed a 
      number of consensus binding sites for both ubiquitous and liver-enriched
      transcription factors. Transfection of human hepatic HepG2 cells with a series of
      5'-deleted promoter luciferase constructs identified a putative silencer element 
      localized in the -1,659/-491 region and a liver-specific positive regulatory
      element localized in the -491/-258 region. This latter region contained the
      liver-abundant transcription factor CCAAT-enhancer binding protein beta
      (C/EBPbeta). The transcriptional activity of the promoter construct containing a 
      mutation in the C/EBPbeta binding site was significantly decreased in HepG2
      cells. This study suggests that C/EBPbeta (-356 to -343) may regulate the liver
      expression of the MRP2 gene.
FAU - Tanaka, T
AU  - Tanaka T
AD  - Department of Biochemistry, Kyushu University School of Medicine, Fukuoka,
      University of Occupational Environmental Health, Kitakyushu, Japan.
FAU - Uchiumi, T
AU  - Uchiumi T
FAU - Hinoshita, E
AU  - Hinoshita E
FAU - Inokuchi, A
AU  - Inokuchi A
FAU - Toh, S
AU  - Toh S
FAU - Wada, M
AU  - Wada M
FAU - Takano, H
AU  - Takano H
FAU - Kohno, K
AU  - Kohno K
FAU - Kuwano, M
AU  - Kuwano M
LA  - eng
SI  - GENBANK/AF144630
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (ABCC2 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Multidrug Resistance-Associated Protein 2)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9EI49ZU76O (multidrug resistance protein 3)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B/chemistry/*genetics/metabolism
MH  - ATP-Binding Cassette Transporters/chemistry/*genetics/metabolism
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - CCAAT-Enhancer-Binding Proteins
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/physiology
MH  - Drug Resistance, Multiple/*genetics
MH  - Gene Expression Regulation/*genetics
MH  - Gene Silencing
MH  - Genes, Reporter
MH  - Genomic Library
MH  - Humans
MH  - Liver/cytology/*metabolism
MH  - Molecular Sequence Data
MH  - Multidrug Resistance-Associated Protein 2
MH  - Nuclear Proteins/physiology
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Response Elements/genetics
MH  - Sequence Deletion/genetics
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0270913999005212 [pii]
AID - 10.1002/hep.510300617 [doi]
PST - ppublish
SO  - Hepatology. 1999 Dec;30(6):1507-12. doi: 10.1002/hep.510300617.