PMID- 10573166
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20200217
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 80 ( Pt 10)
DP  - 1999 Oct
TI  - The rat cytomegalovirus R32 gene encodes a virion-associated protein that elicits
      a strong humoral immune response in infected rats.
PG  - 2719-2728
LID - 10.1099/0022-1317-80-10-2719 [doi]
AB  - A gene of rat cytomegalovirus (RCMV), designated R32, has been identified that
      encodes a homologue of the human cytomegalovirus (HCMV) pp150 (ppUL32) major
      tegument phosphoprotein. The R32 ORF has the capacity to encode a 667 amino acid 
      polypeptide (pR32) with a calculated molecular mass of 73 kDa. The predicted
      amino acid sequence of pR32 shows similarity to that of polypeptides predicted to
      be encoded by the HCMV UL32, murine cytomegalovirus M32 and human herpesvirus
      types 6 and 7 U11 genes. The R32 gene is transcribed as a 2.5 kb mRNA during the 
      late phase of RCMV infection in rat embryo fibroblasts in vitro. To study
      expression of the pR32 protein in vitro and in vivo, a rabbit polyclonal
      antiserum was raised against a recombinant protein that comprised amino acids
      252-522 of pR32. By using this antiserum, pR32 could be detected predominantly in
      the cytoplasm of RCMV-infected fibroblasts at 24 and 48 h post-infection in
      vitro. The pR32 protein was also detected within virions isolated from the
      culture medium of RCMV-infected cells. Expression of pR32 in vivo was observed
      within the cytoplasm of salivary gland epithelial cells of RCMV-infected rats. In
      addition, recombinant pR32 was found to react with sera from rats that were
      previously infected with RCMV, whereas reactivity was not seen with sera from
      mock-infected rats. Together, these findings indicate that RCMV pR32 represents
      the homologue of HCMV ppUL32, both in primary structure and in function.
FAU - Beuken, Erik
AU  - Beuken E
AD  - Department of Medical Microbiology, Cardiovascular Research Institute Maastricht,
      Maastricht University, PO Box 5800, 6202 AZ Maastricht, The Netherlands1.
FAU - Grauls, Gert
AU  - Grauls G
AD  - Department of Medical Microbiology, Cardiovascular Research Institute Maastricht,
      Maastricht University, PO Box 5800, 6202 AZ Maastricht, The Netherlands1.
FAU - Bruggeman, Cathrien A
AU  - Bruggeman CA
AD  - Department of Medical Microbiology, Cardiovascular Research Institute Maastricht,
      Maastricht University, PO Box 5800, 6202 AZ Maastricht, The Netherlands1.
FAU - Vink, Cornelis
AU  - Vink C
AD  - Department of Medical Microbiology, Cardiovascular Research Institute Maastricht,
      Maastricht University, PO Box 5800, 6202 AZ Maastricht, The Netherlands1.
LA  - eng
SI  - GENBANK/U91788
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (pp150 protein, Cytomegalovirus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/biosynthesis
MH  - Antibody Formation
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cytomegalovirus/*genetics/immunology
MH  - Cytomegalovirus Infections/immunology/virology
MH  - DNA, Viral
MH  - Gene Expression
MH  - Genes, Viral
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Phosphoproteins/genetics/immunology
MH  - Rabbits
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Salivary Glands/virology
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
MH  - Viral Matrix Proteins/genetics/*immunology
MH  - Virion
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1099/0022-1317-80-10-2719 [doi]
PST - ppublish
SO  - J Gen Virol. 1999 Oct;80 ( Pt 10):2719-2728. doi: 10.1099/0022-1317-80-10-2719.