PMID- 10571241
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20190822
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 24
IP  - 2
DP  - 1999 Oct
TI  - Direct binding of Reelin to VLDL receptor and ApoE receptor 2 induces tyrosine
      phosphorylation of disabled-1 and modulates tau phosphorylation.
PG  - 481-9
AB  - The large extracellular matrix protein Reelin is produced by Cajal-Retzius
      neurons in specific regions of the developing brain, where it controls neuronal
      migration and positioning. Genetic evidence suggests that interpretation of the
      Reelin signal by migrating neurons involves two neuronal cell surface proteins,
      the very low density lipoprotein receptor (VLDLR) and the apoE receptor 2
      (ApoER2) as well as a cytosolic adaptor protein, Disabled-1 (Dab1). We show that 
      Reelin binds directly and specifically to the ectodomains of VLDLR and ApoER2 in 
      vitro and that blockade of VLDLR and ApoER2 correlates with loss of
      Reelin-induced tyrosine phosphorylation of Disabled-1 in cultured primary
      embryonic neurons. Furthermore, mice that lack either Reelin or both VLDLR and
      ApoER2 exhibit hyperphosphorylation of the microtubule-stabilizing protein tau.
      Taken together, these findings suggest that Reelin acts via VLDLR and ApoER2 to
      regulate Disabled-1 tyrosine phosphorylation and microtubule function in neurons.
FAU - Hiesberger, T
AU  - Hiesberger T
AD  - Department of Molecular Genetics, UT Southwestern Dallas, Texas 75235, USA.
FAU - Trommsdorff, M
AU  - Trommsdorff M
FAU - Howell, B W
AU  - Howell BW
FAU - Goffinet, A
AU  - Goffinet A
FAU - Mumby, M C
AU  - Mumby MC
FAU - Cooper, J A
AU  - Cooper JA
FAU - Herz, J
AU  - Herz J
LA  - eng
GR  - R37 HL063762/HL/NHLBI NIH HHS/United States
GR  - CA41072/CA/NCI NIH HHS/United States
GR  - GM49505/GM/NIGMS NIH HHS/United States
GR  - HL20948/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Dab1 protein, mouse)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Low Density Lipoprotein Receptor-Related Protein-1)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, LDL)
RN  - 0 (Receptors, Lipoprotein)
RN  - 0 (VLDL receptor)
RN  - 0 (tau Proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (reelin protein)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules, Neuronal/genetics/*metabolism
MH  - Extracellular Matrix Proteins/genetics/*metabolism
MH  - Humans
MH  - Low Density Lipoprotein Receptor-Related Protein-1
MH  - Mice
MH  - Mice, Knockout/genetics
MH  - Nerve Tissue Proteins/*metabolism
MH  - Phosphorylation
MH  - Protein Isoforms/metabolism
MH  - Receptors, LDL/genetics/*metabolism
MH  - Receptors, Lipoprotein/genetics/*metabolism
MH  - Serine Endopeptidases
MH  - Tyrosine/*metabolism
MH  - tau Proteins/*metabolism
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0896-6273(00)80861-2 [pii]
AID - 10.1016/s0896-6273(00)80861-2 [doi]
PST - ppublish
SO  - Neuron. 1999 Oct;24(2):481-9. doi: 10.1016/s0896-6273(00)80861-2.