PMID- 10571231
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20190822
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 24
IP  - 2
DP  - 1999 Oct
TI  - A phospho-switch controls the dynamic association of synapsins with synaptic
      vesicles.
PG  - 377-87
AB  - Synapsins constitute a family of synaptic vesicle proteins essential for
      regulating neurotransmitter release. Only two domains are conserved in all
      synapsins: a short N-terminal A domain with a single phosphorylation site for
      cAMP-dependent protein kinase (PKA) and CaM Kinase I, and a large central C
      domain that binds ATP and may be enzymatic. We now demonstrate that synapsin
      phosphorylation in the A domain, at the only phosphorylation site shared by all
      synapsins, dissociates synapsins from synaptic vesicles. Furthermore, we show
      that the A domain binds phospholipids and is inhibited by phosphorylation. Our
      results suggest a novel mechanism by which proteins reversibly bind to membranes 
      using a phosphorylation-dependent phospholipid-binding domain. The dynamic
      association of synapsins with synaptic vesicles correlates with their role in
      activity-dependent plasticity.
FAU - Hosaka, M
AU  - Hosaka M
AD  - Department of Molecular Genetics, Howard Hughes Medical Institute, The University
      of Texas Southwestern Medical School, Dallas 75235, USA.
FAU - Hammer, R E
AU  - Hammer RE
FAU - Sudhof, T C
AU  - Sudhof TC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Phospholipids)
RN  - 0 (Synapsins)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Enzyme Activation/physiology
MH  - Exocytosis/physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phospholipids/metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Substrate Specificity
MH  - Synapsins/genetics/*metabolism
MH  - Synaptic Vesicles/*metabolism
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0896-6273(00)80851-X [pii]
AID - 10.1016/s0896-6273(00)80851-x [doi]
PST - ppublish
SO  - Neuron. 1999 Oct;24(2):377-87. doi: 10.1016/s0896-6273(00)80851-x.