PMID- 10571183
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20220129
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 99
IP  - 4
DP  - 1999 Nov 12
TI  - Targeted mutagenesis of the endogenous mouse Mis gene promoter: in vivo
      definition of genetic pathways of vertebrate sexual development.
PG  - 409-19
AB  - Mutations were introduced into conserved steroidogenic factor 1 (SF1)- and
      SOX9-binding sites within the endogenous mouse Mullerian inhibiting substance
      (Mis) promoter. Male mice homozygous for the mutant SF1-binding site correctly
      initiated Mis transcription in fetal testes, although at significantly reduced
      levels. Surprisingly, sufficient MIS was produced to eliminate the MUllerian
      ducts. In contrast, males homozygous for the mutant SOX9-binding site did not
      initiate Mis transcription, resulting in pseudohermaphrodites. These studies
      suggest an essential role for SOX9 in the initiation of Mis transcription,
      whereas SF1 appears to act as a quantitative regulator of Mis transcript levels, 
      perhaps for influencing non-Mullerian duct tissues. Comparative studies of Mis
      expression in vertebrates indicate that the Mis promoter receives transcriptional
      inputs that vary between species but result in the same functional readout.
FAU - Arango, N A
AU  - Arango NA
AD  - Department of Molecular Genetics, University of Texas M.D. Anderson Cancer
      Center, Houston 77030, USA.
FAU - Lovell-Badge, R
AU  - Lovell-Badge R
FAU - Behringer, R R
AU  - Behringer RR
LA  - eng
GR  - MC_U117562207/MRC_/Medical Research Council/United Kingdom
GR  - HD30284/HD/NICHD NIH HHS/United States
GR  - CA09299/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fushi Tarazu Transcription Factors)
RN  - 0 (Glycoproteins)
RN  - 0 (Growth Inhibitors)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (Sox9 protein, mouse)
RN  - 0 (Steroidogenic Factor 1)
RN  - 0 (Testicular Hormones)
RN  - 0 (Transcription Factors)
RN  - 0 (steroidogenic factor 1, mouse)
RN  - 80497-65-0 (Anti-Mullerian Hormone)
SB  - IM
MH  - Animals
MH  - Anti-Mullerian Hormone
MH  - Binding Sites
MH  - DNA-Binding Proteins/*metabolism
MH  - Female
MH  - Fushi Tarazu Transcription Factors
MH  - Gene Targeting
MH  - *Glycoproteins
MH  - Growth Inhibitors/*genetics/physiology
MH  - High Mobility Group Proteins/*metabolism
MH  - Homeodomain Proteins
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Mullerian Ducts/physiology
MH  - Mutagenesis
MH  - *Promoter Regions, Genetic
MH  - Receptors, Cytoplasmic and Nuclear
MH  - SOX9 Transcription Factor
MH  - Sexual Maturation/*physiology
MH  - Steroidogenic Factor 1
MH  - Testicular Hormones/*genetics/physiology
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
MH  - Up-Regulation
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0092-8674(00)81527-5 [pii]
AID - 10.1016/s0092-8674(00)81527-5 [doi]
PST - ppublish
SO  - Cell. 1999 Nov 12;99(4):409-19. doi: 10.1016/s0092-8674(00)81527-5.