PMID- 10571069
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 460
IP  - 1
DP  - 1999 Oct 22
TI  - Cloning of mouse mitochondrial thymidine kinase 2 cDNA.
PG  - 103-6
AB  - Phosphorylation of anti-viral nucleoside analogs by mitochondrial thymidine
      kinase 2 (TK2) has been implicated as a mechanism for the mitochondrial toxicity 
      caused by several of these compounds. We have cloned the cDNA of mouse TK2 and
      shown that the enzyme is targeted to the mitochondria when expressed in fusion
      with the green fluorescent protein. This is the first report on the cloning of a 
      mitochondrial TK2 and will contribute to elucidate the role of TK2 in the
      pharmacological activation of nucleoside analogs.
FAU - Wettin, K
AU  - Wettin K
AD  - Division of Clinical Virology, Karolinska Institute, Huddinge University
      Hospital, Stockholm, Sweden.
FAU - Johansson, M
AU  - Johansson M
FAU - Zheng, X
AU  - Zheng X
FAU - Zhu, C
AU  - Zhu C
FAU - Karlsson, A
AU  - Karlsson A
LA  - eng
SI  - GENBANK/AF105217
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nucleosides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.1.- (thymidine kinase 2)
RN  - EC 2.7.1.21 (Thymidine Kinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Green Fluorescent Proteins
MH  - Luminescent Proteins
MH  - Mice
MH  - Mitochondria/*enzymology
MH  - Molecular Sequence Data
MH  - Nucleosides/metabolism
MH  - Phosphorylation
MH  - Plasmids
MH  - Recombinant Fusion Proteins/chemistry
MH  - Sequence Alignment
MH  - Thymidine Kinase/chemistry/*genetics
MH  - Transfection
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0014-5793(99)01325-3 [pii]
AID - 10.1016/s0014-5793(99)01325-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Oct 22;460(1):103-6. doi: 10.1016/s0014-5793(99)01325-3.