PMID- 10571041
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 239
IP  - 1
DP  - 1999 Oct 18
TI  - A novel mouse kinesin of the UNC-104/KIF1 subfamily encoded by the Kif1b gene.
PG  - 117-27
AB  - Kinesin and kinesin-related proteins are microtubule-dependent motor proteins
      that transport organelles. We have cloned and sequenced a full-length 9924 bp
      mouse cDNA for a new kinesin of the UNC-104/KIF1 subfamily. Northern blot
      analysis of mouse RNAs detected high levels of a 10 kb mRNA in brain and eye, but
      lower levels in other tissues. Human RNA dot-blot analysis detected this mRNA in 
      all tissues examined, although at different levels. The overall structure of the 
      new kinesin (predicted size 204 kDa) was most similar to mouse KIF1A; however,
      2.1 kb of the 5' portion of the cDNA were identical to the published sequence for
      KIF1B (Nangaku, M., Sato-Yoshitake, R., Okada, Y., Noda, Y., Takemura, R.,
      Yamazaki, H., Hirokawa, N., 1994. KIF1B, a novel microtubule plus end-directed
      monomeric motor protein for transport of mitochondria. Cell 79, 1209-1220). We
      localized the Kif1b gene to the distal end of mouse Chromosome 4 by haplotype
      analysis of an interspecific backcross from The Jackson Laboratory. We had
      previously mapped the gene for the novel kinesin to the same location (Gong,
      T.-W.L., Burmeister, M., Lomax, M.I., 1996b. The novel gene D4Mille maps to mouse
      Chromosome 4 and human Chromosome 1p36. Mamm. Genome 7, 790-791). We conclude,
      therefore, that the Kif1b gene generates two major kinesin isoforms by
      alternative splicing. The shorter 7.8 kb mRNA encodes a 130 kDa kinesin,
      KIF1Bp130, whereas the 10 kb mRNA encodes a 204 kDa kinesin, KIF1Bp204. In
      addition, alternative splicing of two exons in the conserved region adjacent to
      the motor domain generates four different isoforms of each kinesin, leading to
      eight kinesin isoforms derived from the Kif1b gene.
FAU - Gong, T W
AU  - Gong TW
AD  - Kresge Hearing Research Institute, Department of Otolaryngology/Head Neck
      Surgery, University of Michigan, Ann Arbor 48109, USA.
FAU - Winnicki, R S
AU  - Winnicki RS
FAU - Kohrman, D C
AU  - Kohrman DC
FAU - Lomax, M I
AU  - Lomax MI
LA  - eng
SI  - GENBANK/AF090190
GR  - DC02492/DC/NIDCD NIH HHS/United States
GR  - DC02982/DC/NIDCD NIH HHS/United States
GR  - DC03049/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (KIF1B protein, human)
RN  - 0 (Kif1b protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.4.4 (Kinesin)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chickens
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Female
MH  - Gene Expression
MH  - Genetic Variation
MH  - Humans
MH  - Kinesin/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Muridae
MH  - Nerve Tissue Proteins/*genetics
MH  - Protein Isoforms/genetics
MH  - Protein Structure, Secondary
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0378-1119(99)00370-4 [pii]
AID - 10.1016/s0378-1119(99)00370-4 [doi]
PST - ppublish
SO  - Gene. 1999 Oct 18;239(1):117-27. doi: 10.1016/s0378-1119(99)00370-4.