PMID- 10570975
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20191224
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 238
IP  - 2
DP  - 1999 Oct 1
TI  - Nephroblastoma overexpressed gene (NOV) codes for a growth factor that induces
      protein tyrosine phosphorylation.
PG  - 471-8
AB  - NOV (nephroblastoma overexpressed gene) is a member of the CCN (connective tissue
      growth factor [CTGF], Cyr61/Cef10, NOV) family of proteins. These proteins are
      cysteine-rich and are noted for having growth-regulatory functions. We have
      isolated the rat NOV gene, and the DNA sequence shares 90% identity with the
      mouse and 80% identity with the human sequences. The rat NOV gene was expressed
      in all rat tissues examined, including brain, lung, heart, kidney, liver, spleen,
      thymus and skeletal muscle. Higher levels of rat NOV mRNA were seen in the brain,
      lung and skeletal muscle compared to the other tissues. Examination of NOV
      expression in various human cell lines revealed that NOV was expressed in U87,
      293, T98G, SK-N-MC and Hs683 but not in HepG2, HL60, THP1 and Jurkat. The human
      NOV gene was transfected into 293 cells and the expressed protein purified. When 
      3T3 fibroblasts were treated with this recombinant NOV protein, a dose-dependent 
      increase in proliferation was observed. Analysis of tyrosine-phosphorylated
      proteins revealed that when 3T3 cells were treated with NOV, a 221 kDa protein
      was phosphorylated. These data suggest that NOV can act as a growth factor for
      some cells and binds to a specific receptor that leads to the phosphorylation of 
      a 221 kDa protein.
FAU - Liu, C
AU  - Liu C
AD  - Neurocrine Biosciences Inc., San Diego, CA 92121, USA. cliu@neurocrine.com
FAU - Liu, X J
AU  - Liu XJ
FAU - Crowe, P D
AU  - Crowe PD
FAU - Kelner, G S
AU  - Kelner GS
FAU - Fan, J
AU  - Fan J
FAU - Barry, G
AU  - Barry G
FAU - Manu, F
AU  - Manu F
FAU - Ling, N
AU  - Ling N
FAU - De Souza, E B
AU  - De Souza EB
FAU - Maki, R A
AU  - Maki RA
LA  - eng
SI  - GENBANK/AF171936
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (CCN2 protein, human)
RN  - 0 (CCN2 protein, mouse)
RN  - 0 (CCN2 protein, rat)
RN  - 0 (CCN3 protein, human)
RN  - 0 (Ccn3 protein, mouse)
RN  - 0 (DNA, Complementary)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Nephroblastoma Overexpressed Protein)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - 42HK56048U (Tyrosine)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Division
MH  - Cloning, Molecular
MH  - Connective Tissue Growth Factor
MH  - DNA, Complementary
MH  - Dexamethasone/pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - *Immediate-Early Proteins
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Kidney Neoplasms/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nephroblastoma Overexpressed Protein
MH  - Oncogene Proteins, Viral/*genetics/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins/*genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
MH  - Tyrosine/*metabolism
MH  - Wilms Tumor/*genetics
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0378111999003649 [pii]
AID - 10.1016/s0378-1119(99)00364-9 [doi]
PST - ppublish
SO  - Gene. 1999 Oct 1;238(2):471-8. doi: 10.1016/s0378-1119(99)00364-9.