PMID- 10570971
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20220309
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 238
IP  - 2
DP  - 1999 Oct 1
TI  - Identification and characterization of Drosophila homolog of Rho-kinase.
PG  - 437-44
AB  - The Rho family of small GTPases and their associated regulators and targets are
      essential mediators of diverse morphogenetic events in development. Mammalian
      Rho-kinase/ROK alpha, one of the targets of Rho, has been shown to bind to Rho in
      GTP-bound form and to phosphorylate the myosin light chain (MLC) and the
      myosin-binding subunit (MBS) of myosin phosphatase, resulting in the activation
      of myosin. Thus, Rho-kinase/ROK alpha has been suggested to play essential roles 
      in the formation of stress fibers and focal adhesions. We have identified the
      Drosophila homolog of Rho-kinase/ROK alpha, DRho-kinase, which has conserved the 
      basic structural feature of Rho-kinase/ROK alpha consisting of the N-terminal
      kinase, central coiled-coil and C-terminal pleckstrin homology (PH) domains. A
      two-hybrid analysis demonstrated that DRho-kinase interacts with the GTP-bound
      form of the Drosophila Rho. Drho1, at the conserved Rho-binding site. DRho-kinase
      can phosphorylate MLC and MBS, preferable substrates for bovine Rho-kinase, in
      vitro. DRho-kinase is ubiquitously expressed throughout development, in a pattern
      essentially identical to that of Drho1. These results suggest that DRho-kinase is
      an effector of Drho1.
FAU - Mizuno, T
AU  - Mizuno T
AD  - Division of Biological Science, Graduate School of Science, Nagoya University,
      Japan.
FAU - Amano, M
AU  - Amano M
FAU - Kaibuchi, K
AU  - Kaibuchi K
FAU - Nishida, Y
AU  - Nishida Y
LA  - eng
SI  - GENBANK/AF151375
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Drosophila/*enzymology
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Serine-Threonine Kinases/*chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - rho-Associated Kinases
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0378111999003510 [pii]
AID - 10.1016/s0378-1119(99)00351-0 [doi]
PST - ppublish
SO  - Gene. 1999 Oct 1;238(2):437-44. doi: 10.1016/s0378-1119(99)00351-0.