PMID- 10570961
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 238
IP  - 2
DP  - 1999 Oct 1
TI  - Hsc40, a new member of the hsp40 family, exhibits similar expression profile to
      that of hsc70 in mammalian cells.
PG  - 333-41
AB  - Here, we report the identification and characterization of a new hsp40 family
      member, heat shock cognate 40 (hsc40), which may be a specific functional partner
      for hsc70. The hsc40 gene consists of five exons and four introns. The previously
      characterized hsp40 gene contains only three exons and two introns. Despite this 
      difference in the numbers of exons and introns, the structures of these two genes
      are actually quite similar. If the first two exons and introns of the hsc40 gene 
      are excluded, the rest is essentially the same as the whole hsp40 gene. The
      intron/exon boundaries of the hsc40 and hsp40 genes are conserved with respect to
      amino acid coding, indicating that the hsp40 gene might have arisen from a
      duplicated hsc40 gene, which developed a new transcription-regulatory mechanism
      in the second intron. Hsc40 is evolutionally conserved. There is over 95%
      sequence identity between the putative mouse and human hsc40 proteins. However,
      there is only 60% sequence identity between the hsc40 and hsp40 proteins from
      either human or mouse cells. Northern blot analysis of various tissues and cells 
      in culture revealed that this gene was expressed under normal conditions, and its
      expression was further increased after various stress treatments. The expression 
      pattern of the hsc40 gene is very similar to that of the hsc70 gene under both
      normal and stress conditions, whereas the hsp40 gene exhibited an expression
      pattern more similar to that of hsp70 genes on the same Northern blots. These
      results, considered along with the high rate of sequence conservation between the
      same proteins from different species and high rate of variation between the two
      different proteins from the same species, strongly suggest that hsc40 and hsp40
      may perform different functions and/or have different specificities in the cell.
FAU - Chen, M S
AU  - Chen MS
AD  - Section of Cancer Biology, Radiation Oncology Center, Washington University
      School of Medicine, St. Louis, MO 63108, USA. mingshun@radonc.wustl.edu
FAU - Roti, J R
AU  - Roti JR
FAU - Laszlo, A
AU  - Laszlo A
LA  - eng
SI  - GENBANK/AF092536
GR  - CA-43198/CA/NCI NIH HHS/United States
GR  - CA-49018/CA/NCI NIH HHS/United States
GR  - CA-75556/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNAJB1 protein, human)
RN  - 0 (DNAJB5 protein, human)
RN  - 0 (Dnajb1 protein, mouse)
RN  - 0 (Dnajb5 protein, mouse)
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 0 (HSP40 Heat-Shock Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSPA8 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Hspa8 protein, mouse)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/*genetics
MH  - DNA, Complementary
MH  - Exons
MH  - Gene Expression Regulation
MH  - HSC70 Heat-Shock Proteins
MH  - HSP40 Heat-Shock Proteins
MH  - *HSP70 Heat-Shock Proteins
MH  - Heat-Shock Proteins/chemistry/*genetics
MH  - Heat-Shock Response
MH  - Humans
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription, Genetic
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0378111999003339 [pii]
AID - 10.1016/s0378-1119(99)00333-9 [doi]
PST - ppublish
SO  - Gene. 1999 Oct 1;238(2):333-41. doi: 10.1016/s0378-1119(99)00333-9.