PMID- 10570482
OWN - NLM
STAT- MEDLINE
DCOM- 20000118
LR  - 20171116
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 2
IP  - 12
DP  - 1999 Dec
TI  - Microtubule binding by CRIPT and its potential role in the synaptic clustering of
      PSD-95.
PG  - 1063-9
AB  - CRIPT is a postsynaptic protein that binds selectively to the third PDZ domain
      (PDZ3) of PSD-95. Here we show that CRIPT also binds directly to microtubules,
      thereby linking PSD-95 to the microtubule cytoskeleton. Disrupting the
      CRIPT-PSD-95 interaction in cultured hippocampal neurons with a PDZ3-specific
      peptide prevented the association of PSD-95 with microtubules and inhibited the
      synaptic clustering of PSD-95, chapsyn-110/PSD-93 and GKAP (a PSD-95-binding
      protein). However, the number of synapses and the synaptic clustering of NMDA
      receptors were unaffected, suggesting that PSD-95-family proteins are not
      essential for the maintenance of synapses and the synaptic localization of NMDA
      receptors.
FAU - Passafaro, M
AU  - Passafaro M
AD  - Howard Hughes Medical Institute and Department of Neurobiology, Massachusetts
      General Hospital and Harvard Medical School, HHMI/Wellman 423, 50 Blossom Street,
      Boston, Massachusetts 02114, USA.
FAU - Sala, C
AU  - Sala C
FAU - Niethammer, M
AU  - Niethammer M
FAU - Sheng, M
AU  - Sheng M
LA  - eng
GR  - NS35050/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SAP90-PSD95 Associated Proteins)
RN  - 0 (Synaptophysin)
RN  - 0 (postsynaptic density proteins)
RN  - EC 2.7.11.1 (CASK kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.4.4 (Nucleoside-Phosphate Kinase)
RN  - EC 2.7.4.8 (Guanylate Kinases)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - COS Cells
MH  - *Calcium-Calmodulin-Dependent Protein Kinases
MH  - Carrier Proteins/antagonists & inhibitors/chemistry/genetics/*metabolism
MH  - Cells, Cultured
MH  - Guanylate Kinases
MH  - Hippocampus/cytology
MH  - Membrane Proteins/chemistry/genetics/*metabolism
MH  - Microtubule-Associated Proteins/metabolism
MH  - Microtubules/drug effects/*metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/antagonists & inhibitors/chemistry/genetics/*metabolism
MH  - Neurons/cytology/drug effects/enzymology/metabolism
MH  - Nucleoside-Phosphate Kinase/antagonists & inhibitors/metabolism
MH  - Peptide Fragments/chemistry/metabolism/pharmacology
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Recombinant Fusion Proteins/chemistry/metabolism/pharmacology
MH  - SAP90-PSD95 Associated Proteins
MH  - Synapses/drug effects/enzymology/*metabolism
MH  - Synaptophysin/metabolism
EDAT- 1999/11/26 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/11/26 09:00
PHST- 1999/11/26 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/11/26 09:00 [entrez]
AID - 10.1038/15990 [doi]
PST - ppublish
SO  - Nat Neurosci. 1999 Dec;2(12):1063-9. doi: 10.1038/15990.