PMID- 10570481
OWN - NLM
STAT- MEDLINE
DCOM- 20000118
LR  - 20161124
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 2
IP  - 12
DP  - 1999 Dec
TI  - Ggamma13 colocalizes with gustducin in taste receptor cells and mediates IP3
      responses to bitter denatonium.
PG  - 1055-62
AB  - Gustducin is a transducin-like G protein selectively expressed in taste receptor 
      cells. The alpha subunit of gustducin (alpha-gustducin) is critical for
      transduction of responses to bitter or sweet compounds. We identified a G-protein
      gamma subunit (Ggamma13) that colocalized with alpha-gustducin in taste receptor 
      cells. Of 19 alpha-gustducin/Ggamma13-positive taste receptor cells profiled, all
      expressed the G protein beta3 subunit (Gbeta3); approximately 80% also expressed 
      Gbeta1. Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13) were activated 
      by taste cell membranes plus bitter denatonium. Antibodies against Ggamma13
      blocked the denatonium-induced increase of inositol trisphosphate (IP3) in taste 
      tissue. We conclude that gustducin heterotrimers transduce responses to bitter
      and sweet compounds via alpha-gustducin's regulation of phosphodiesterase (PDE)
      and Gbetagamma's activation of phospholipase C (PLC).
FAU - Huang, L
AU  - Huang L
AD  - Howard Hughes Medical Institute, Mount Sinai School of Medicine of New York
      University, Box 1677, One Gustave L. Levy Place, New York, New York 10029, USA.
FAU - Shanker, Y G
AU  - Shanker YG
FAU - Dubauskaite, J
AU  - Dubauskaite J
FAU - Zheng, J Z
AU  - Zheng JZ
FAU - Yan, W
AU  - Yan W
FAU - Rosenzweig, S
AU  - Rosenzweig S
FAU - Spielman, A I
AU  - Spielman AI
FAU - Max, M
AU  - Max M
FAU - Margolskee, R F
AU  - Margolskee RF
LA  - eng
GR  - DC03155/DC/NIDCD NIH HHS/United States
GR  - DE10754/DE/NIDCR NIH HHS/United States
GR  - MH57241/MH/NIMH NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (G-protein Beta gamma)
RN  - 0 (GTP-Binding Protein beta Subunits)
RN  - 0 (GTP-Binding Protein gamma Subunits)
RN  - 0 (Isoenzymes)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Messenger)
RN  - 147979-21-3 (gustducin)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.11 (PLCB3 protein, human)
RN  - EC 3.1.4.11 (Phospholipase C beta)
RN  - EC 3.1.4.11 (Plcb3 protein, mouse)
RN  - EC 3.1.4.11 (Plcb3 protein, rat)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins)
RN  - EC 3.6.5.1 (Transducin)
RN  - G2NT7SMW7M (denatonium saccharide)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - Enzyme Activation
MH  - Epithelial Cells/chemistry
MH  - *GTP-Binding Protein beta Subunits
MH  - *GTP-Binding Protein gamma Subunits
MH  - GTP-Binding Proteins/analysis/genetics/*metabolism
MH  - Gene Expression Profiling
MH  - *Heterotrimeric GTP-Binding Proteins
MH  - Humans
MH  - Inositol 1,4,5-Trisphosphate/*metabolism
MH  - Isoenzymes/metabolism
MH  - Lingual Frenum
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Phospholipase C beta
MH  - Phosphoric Diester Hydrolases/metabolism
MH  - Protein Binding
MH  - Quaternary Ammonium Compounds/*pharmacology
MH  - RNA, Messenger/analysis/genetics
MH  - Sequence Alignment
MH  - Taste/*physiology
MH  - Taste Buds/*chemistry/cytology/enzymology/metabolism
MH  - Transducin/analysis/chemistry/genetics/*metabolism
MH  - Type C Phospholipases/metabolism
EDAT- 1999/11/26 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/11/26 09:00
PHST- 1999/11/26 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/11/26 09:00 [entrez]
AID - 10.1038/15981 [doi]
PST - ppublish
SO  - Nat Neurosci. 1999 Dec;2(12):1055-62. doi: 10.1038/15981.