PMID- 10570256
OWN - NLM
STAT- MEDLINE
DCOM- 19991220
LR  - 20190826
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 11
DP  - 1999 Dec 1
TI  - Cutting edge: SLP-76 cooperativity with FYB/FYN-T in the Up-regulation of
      TCR-driven IL-2 transcription requires SLP-76 binding to FYB at Tyr595 and
      Tyr651.
PG  - 5753-7
AB  - SLP-76 (Src homology (SH) 2-domain-containing leukocyte protein of 76 kDa) and
      FYB/SLAP (FYN-T-binding protein/SLP-76-associated protein) are two hemopoietic
      cell-specific adaptor proteins downstream of TCR-activated protein tyrosine
      kinases. SLP-76 has been implicated as an essential component in T cell
      signaling. FYB is selectively phosphorylated by FYN-T, providing a template for
      the recruitment of FYN-T and SLP-76 SH2 domains. Coexpression of FYN-T, FYB, and 
      SLP-76 can synergistically up-regulate IL-2 production in T cells upon TCR
      ligation. In this report, we show that two tyrosines, Tyr595 and Tyr651, of FYB
      are major sites of phosphorylation by FYN-T and mediate binding to SLP-76 in
      Jurkat T cells. Furthermore, the synergistic up-regulation of IL-2 promoter
      activity in the FYN-T-FYB-SLP-76 pathway is contingent upon the interaction
      between FYB and SLP-76, but not the interaction between FYB and FYN-T. These
      observations define a pathway by which SLP-76 interacts with downstream
      components in the up-regulation of T cell cytokine production.
FAU - Geng, L
AU  - Geng L
AD  - Division of Tumor Immunology, Department of Cancer Immunology and AIDS, Harvard
      Medical School, Boston, MA 02115, USA.
FAU - Raab, M
AU  - Raab M
FAU - Rudd, C E
AU  - Rudd CE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (FYB1 protein, human)
RN  - 0 (Interleukin-2)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (SLP-76 signal Transducing adaptor proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-fyn)
SB  - AIM
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Carrier Proteins/*metabolism
MH  - Interleukin-2/*biosynthesis
MH  - Molecular Sequence Data
MH  - Phosphoproteins/*metabolism
MH  - Phosphorylation
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-fyn
MH  - Receptors, Antigen, T-Cell/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - Up-Regulation
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - ji_v163n11p5753 [pii]
PST - ppublish
SO  - J Immunol. 1999 Dec 1;163(11):5753-7.