PMID- 10570151
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 24
DP  - 1999 Nov 23
TI  - Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing.
PG  - 13789-94
AB  - The conserved CDC5 family of Myb-related proteins performs an essential function 
      in cell cycle control at G(2)/M. Although c-Myb and many Myb-related proteins act
      as transcription factors, herein, we implicate CDC5 proteins in pre-mRNA
      splicing. Mammalian CDC5 colocalizes with pre-mRNA splicing factors in the nuclei
      of mammalian cells, associates with core components of the splicing machinery in 
      nuclear extracts, and interacts with the spliceosome throughout the splicing
      reaction in vitro. Furthermore, genetic depletion of the homolog of CDC5 in
      Saccharomyces cerevisiae, CEF1, blocks the first step of pre-mRNA processing in
      vivo. These data provide evidence that eukaryotic cells require CDC5 proteins for
      pre-mRNA splicing.
FAU - Burns, C G
AU  - Burns CG
AD  - Department of Cell Biology, Vanderbilt University, School of Medicine, Nashville,
      TN 37232, USA. geoff.burns@mcmail.vanderbilt.edu
FAU - Ohi, R
AU  - Ohi R
FAU - Krainer, A R
AU  - Krainer AR
FAU - Gould, K L
AU  - Gould KL
LA  - eng
GR  - T32 GM007347/GM/NIGMS NIH HHS/United States
GR  - GM47728/GM/NIGMS NIH HHS/United States
GR  - P30 CA068485/CA/NCI NIH HHS/United States
GR  - P01 CA013106/CA/NCI NIH HHS/United States
GR  - CA68485/CA/NCI NIH HHS/United States
GR  - DK20593/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CEF1 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myb)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Schizosaccharomyces pombe Proteins)
RN  - 0 (cdc5 protein, S pombe)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (polo-like kinase 1)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Fungal Proteins/genetics/*metabolism
MH  - Humans
MH  - Mice
MH  - Protein Kinases/genetics/*metabolism
MH  - Protein-Serine-Threonine Kinases
MH  - Proto-Oncogene Proteins
MH  - *Proto-Oncogene Proteins c-myb
MH  - *RNA Precursors
MH  - *RNA Splicing
MH  - RNA-Binding Proteins
MH  - Saccharomyces cerevisiae/genetics
MH  - Saccharomyces cerevisiae Proteins
MH  - Schizosaccharomyces
MH  - Schizosaccharomyces pombe Proteins
MH  - Spliceosomes
MH  - Subcellular Fractions
PMC - PMC24143
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1073/pnas.96.24.13789 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13789-94. doi:
      10.1073/pnas.96.24.13789.